6YAN

Mammalian 48S late-stage translation initiation complex with histone 4 mRNA

6YAN の概要

• エントリーDOI: 10.2210/pdb6yan/pdb
• 関連するPDBエントリー: 6YAL 6YAM
• EMDBエントリー: 10762 10763 10764
• 分子名称: 60s ribosomal protein l41, 40S ribosomal protein S8, Ribosomal protein S9 (Predicted), ... (39 entities in total)
• 機能のキーワード: initiation complex, 48s, eukaryotic initiation factor 1a, rabbit, translation
• 由来する生物種: Oryctolagus cuniculus (Rabbit); 詳細
• タンパク質・核酸の鎖数: 39
• 化学式量合計: 1273879.90

構造登録者

Bochler, A.,Simonetti, A.,Guca, E.,Hashem, Y. (登録日: 2020-03-12, 公開日: 2020-04-08, 最終更新日: 2024-10-23)

主引用文献

Simonetti, A.,Guca, E.,Bochler, A.,Kuhn, L.,Hashem, Y.
Structural Insights into the Mammalian Late-Stage Initiation Complexes.
Cell Rep, 31:107497-107497, 2020

PubMed Abstract: In higher eukaryotes, the mRNA sequence in the direct vicinity of the start codon, called the Kozak sequence (CRCCaugG, where R is a purine), is known to influence the rate of the initiation process. However, the molecular basis underlying its role remains poorly understood. Here, we present the cryoelectron microscopy (cryo-EM) structures of mammalian late-stage 48S initiation complexes (LS48S ICs) in the presence of two different native mRNA sequences, β-globin and histone 4, at overall resolution of 3 and 3.5 Å, respectively. Our high-resolution structures unravel key interactions from the mRNA to eukaryotic initiation factors (eIFs): 1A, 2, 3, 18S rRNA, and several 40S ribosomal proteins. In addition, we are able to study the structural role of ABCE1 in the formation of native 48S ICs. Our results reveal a comprehensive map of ribosome/eIF-mRNA and ribosome/eIF-tRNA interactions and suggest the impact of mRNA sequence on the structure of the LS48S IC.

PubMed: 32268096
DOI: 10.1016/j.celrep.2020.03.061

実験手法

ELECTRON MICROSCOPY (3.48 Å)