6Y9O

Crystal structure of Whirlin PDZ3_C-ter in complex with CASK internal PDZ binding motif peptide

6Y9O の概要

• エントリーDOI: 10.2210/pdb6y9o/pdb
• 分子名称: Whirlin, Peripheral plasma membrane protein CASK (3 entities in total)
• 機能のキーワード: whirlin, pdz, myosin 15a, complex, structural protein
• 由来する生物種: Mus musculus (House mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 12791.69

構造登録者

Zhu, Y.,Delhommel, F.,Haouz, A.,Caillet-Saguy, C.,Vaney, M.,Mechaly, A.E.,Wolff, N. (登録日: 2020-03-10, 公開日: 2020-10-07, 最終更新日: 2024-01-24)

主引用文献

Zhu, Y.,Delhommel, F.,Cordier, F.,Luchow, S.,Mechaly, A.,Colcombet-Cazenave, B.,Girault, V.,Pepermans, E.,Bahloul, A.,Gautier, C.,Brule, S.,Raynal, B.,Hoos, S.,Haouz, A.,Caillet-Saguy, C.,Ivarsson, Y.,Wolff, N.
Deciphering the Unexpected Binding Capacity of the Third PDZ Domain of Whirlin to Various Cochlear Hair Cell Partners.
J.Mol.Biol., 432:5920-5937, 2020

PubMed Abstract: Hearing is a mechanical and neurochemical process, which occurs in the hair cells of inner ear that converts the sound vibrations into electrical signals transmitted to the brain. The multi-PDZ scaffolding protein whirlin plays a critical role in the formation and function of stereocilia exposed at the surface of hair cells. In this article, we reported seven stereociliary proteins that encode PDZ binding motifs (PBM) and interact with whirlin PDZ3, where four of them are first reported. We solved the atomic resolution structures of complexes between whirlin PDZ3 and the PBMs of myosin 15a, CASK, harmonin a1 and taperin. Interestingly, the PBM of CASK and taperin are rare non-canonical PBM, which are not localized at the extreme C terminus. This large capacity to accommodate various partners could be related to the distinct functions of whirlin at different stages of the hair cell development.

PubMed: 32971111
DOI: 10.1016/j.jmb.2020.09.012

実験手法

X-RAY DIFFRACTION (1.632 Å)