6Y59

5-HT3A receptor in Salipro (apo, C5 symmetric)

6Y59 の概要

• エントリーDOI: 10.2210/pdb6y59/pdb
• 関連するPDBエントリー: 6Y5A 6Y5B
• EMDBエントリー: 10691 10692 10693
• 分子名称: 5-hydroxytryptamine receptor 3A (1 entity in total)
• 機能のキーワード: pentameric ligand-gated ion channel, plgic, serotonin 5-ht3a receptor, 5-ht3ar, salipro, membrane protein
• 由来する生物種: Mus musculus (House mouse)
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 303340.43

構造登録者

Zhang, Y.,Dijkman, P.M.,Zou, R.,Zandl-Lang, M.,Sanchez, R.M.,Eckhardt-Strelau, L.,Koefeler, H.,Vogel, H.,Yuan, S.,Kudryashev, M. (登録日: 2020-02-25, 公開日: 2020-12-23, 最終更新日: 2024-10-23)

主引用文献

Zhang, Y.,Dijkman, P.M.,Zou, R.,Zandl-Lang, M.,Sanchez, R.M.,Eckhardt-Strelau, L.,Kofeler, H.,Vogel, H.,Yuan, S.,Kudryashev, M.
Asymmetric opening of the homopentameric 5-HT 3A serotonin receptor in lipid bilayers.
Nat Commun, 12:1074-1074, 2021

PubMed Abstract: Pentameric ligand-gated ion channels (pLGICs) of the Cys-loop receptor family are key players in fast signal transduction throughout the nervous system. They have been shown to be modulated by the lipid environment, however the underlying mechanism is not well understood. We report three structures of the Cys-loop 5-HT serotonin receptor (5HTR) reconstituted into saposin-based lipid bilayer discs: a symmetric and an asymmetric apo state, and an asymmetric agonist-bound state. In comparison to previously published 5HTR conformations in detergent, the lipid bilayer stabilises the receptor in a more tightly packed, 'coupled' state, involving a cluster of highly conserved residues. In consequence, the agonist-bound receptor conformation adopts a wide-open pore capable of conducting sodium ions in unbiased molecular dynamics (MD) simulations. Taken together, we provide a structural basis for the modulation of 5HTR by the membrane environment, and a model for asymmetric activation of the receptor.

PubMed: 33594077
DOI: 10.1038/s41467-021-21016-7

実験手法

ELECTRON MICROSCOPY (3.2 Å)