6Y4C

Structure of galectin-3C in complex with lactose determined by serial crystallography using an XtalTool support

6Y4C の概要

• エントリーDOI: 10.2210/pdb6y4c/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900004
• 分子名称: Galectin-3, beta-D-galactopyranose-(1-4)-beta-D-glucopyranose, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: galectin, synchrotron serial crystallography, solid support, xtaltool, sugar binding protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16078.80

構造登録者

Shilova, A.,Hakansson, M.,Welin, M.,Kovacic, R.,Mueller, U.,Logan, D.T. (登録日: 2020-02-20, 公開日: 2020-06-17, 最終更新日: 2024-01-24)

主引用文献

Shilova, A.,Lebrette, H.,Aurelius, O.,Nan, J.,Welin, M.,Kovacic, R.,Ghosh, S.,Safari, C.,Friel, R.J.,Milas, M.,Matej, Z.,Hogbom, M.,Branden, G.,Kloos, M.,Shoeman, R.L.,Doak, B.,Ursby, T.,Hakansson, M.,Logan, D.T.,Mueller, U.
Current status and future opportunities for serial crystallography at MAX IV Laboratory.
J.Synchrotron Radiat., 27:1095-1102, 2020

PubMed Abstract: Over the last decade, serial crystallography, a method to collect complete diffraction datasets from a large number of microcrystals delivered and exposed to an X-ray beam in random orientations at room temperature, has been successfully implemented at X-ray free-electron lasers and synchrotron radiation facility beamlines. This development relies on a growing variety of sample presentation methods, including different fixed target supports, injection methods using gas-dynamic virtual-nozzle injectors and high-viscosity extrusion injectors, and acoustic levitation of droplets, each with unique requirements. In comparison with X-ray free-electron lasers, increased beam time availability makes synchrotron facilities very attractive to perform serial synchrotron X-ray crystallography (SSX) experiments. Within this work, the possibilities to perform SSX at BioMAX, the first macromolecular crystallography beamline at  MAX IV Laboratory in Lund, Sweden, are described, together with case studies from the SSX user program: an implementation of a high-viscosity extrusion injector to perform room temperature serial crystallography at BioMAX using two solid supports - silicon nitride membranes (Silson, UK) and XtalTool (Jena Bioscience, Germany). Future perspectives for the dedicated serial crystallography beamline MicroMAX at MAX IV Laboratory, which will provide parallel and intense micrometre-sized X-ray beams, are discussed.

PubMed: 32876583
DOI: 10.1107/S1600577520008735

実験手法

X-RAY DIFFRACTION (1.7 Å)