6Y45

Crystal Structure of the H33A variant of RsrR

6Y45 の概要

• エントリーDOI: 10.2210/pdb6y45/pdb
• 分子名称: Rrf2 family transcriptional regulator, FE2/S2 (INORGANIC) CLUSTER, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (7 entities in total)
• 機能のキーワード: redox sensor, iron sulfur cluster, transcription
• 由来する生物種: Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 71238.58

構造登録者

Rohac, R.,Volbeda, A.,Fontecilla-Camps, J.C. (登録日: 2020-02-19, 公開日: 2020-03-04, 最終更新日: 2024-01-24)

主引用文献

Crack, J.C.,Amara, P.,Volbeda, A.,Mouesca, J.M.,Rohac, R.,Pellicer Martinez, M.T.,Huang, C.Y.,Gigarel, O.,Rinaldi, C.,Le Brun, N.E.,Fontecilla-Camps, J.C.
Electron and Proton Transfers Modulate DNA Binding by the Transcription Regulator RsrR.
J.Am.Chem.Soc., 142:5104-5116, 2020

PubMed Abstract: The [FeS]-RsrR gene transcription regulator senses the redox status in bacteria by modulating DNA binding, while its cluster cycles between +1 and +2 states-only the latter binds DNA. We have previously shown that RsrR can undergo remarkable conformational changes involving a 100° rotation of tryptophan 9 between exposed () and buried () states. Here, we have used the chemical modification of Trp9, site-directed mutagenesis, and crystallographic and computational chemical studies to show that (i) the and states correspond to oxidized and reduced RsrR, respectively, (ii) His33 is protonated in the state due to a change in its p caused by cluster reduction, and (iii) Trp9 rotation is conditioned by the response of its dipole moment to environmental electrostatic changes. Our findings illustrate a novel function of protonation resulting from electron transfer.

PubMed: 32078310
DOI: 10.1021/jacs.9b12250

実験手法

X-RAY DIFFRACTION (1.68 Å)