6Y1H
Major subunit ComGC from S. pneumoniae Com pseudopili
Summary for 6Y1H
| Entry DOI | 10.2210/pdb6y1h/pdb |
| NMR Information | BMRB: 34490 |
| Descriptor | Competence protein (1 entity in total) |
| Functional Keywords | type iv filaments type iv pilin competence pseudopili mainly alpha orthogonal bundle, structural protein |
| Biological source | Streptococcus pneumoniae R6 |
| Total number of polymer chains | 1 |
| Total formula weight | 7948.80 |
| Authors | Sheppard, D.,Berry, J.L.,Matthews, S.J.,Pelicic, V. (deposition date: 2020-02-12, release date: 2020-04-15, Last modification date: 2024-06-19) |
| Primary citation | Sheppard, D.,Berry, J.L.,Denise, R.,Rocha, E.P.C.,Matthews, S.,Pelicic, V. The major subunit of widespread competence pili exhibits a novel and conserved type IV pilin fold. J.Biol.Chem., 295:6594-6604, 2020 Cited by PubMed Abstract: Type IV filaments (T4F), which are helical assemblies of type IV pilins, constitute a superfamily of filamentous nanomachines virtually ubiquitous in prokaryotes that mediate a wide variety of functions. The competence (Com) pilus is a widespread T4F, mediating DNA uptake (the first step in natural transformation) in bacteria with one membrane (monoderms), an important mechanism of horizontal gene transfer. Here, we report the results of genomic, phylogenetic, and structural analyses of ComGC, the major pilin subunit of Com pili. By performing a global comparative analysis, we show that Com pili genes are virtually ubiquitous in Bacilli, a major monoderm class of Firmicutes. This also revealed that ComGC displays extensive sequence conservation, defining a monophyletic group among type IV pilins. We further report ComGC solution structures from two naturally competent human pathogens, (ComGC) and (ComGC), revealing that this pilin displays extensive structural conservation. Strikingly, ComGC and ComGC exhibit a novel type IV pilin fold that is purely helical. Results from homology modeling analyses suggest that the unusual structure of ComGC is compatible with helical filament assembly. Because ComGC displays such a widespread distribution, these results have implications for hundreds of monoderm species. PubMed: 32273343DOI: 10.1074/jbc.RA120.013316 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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