6Y1H

Major subunit ComGC from S. pneumoniae Com pseudopili

6Y1H の概要

• エントリーDOI: 10.2210/pdb6y1h/pdb
• NMR情報: BMRB: 34490
• 分子名称: Competence protein (1 entity in total)
• 機能のキーワード: type iv filaments type iv pilin competence pseudopili mainly alpha orthogonal bundle, structural protein
• 由来する生物種: Streptococcus pneumoniae R6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7948.80

構造登録者

Sheppard, D.,Berry, J.L.,Matthews, S.J.,Pelicic, V. (登録日: 2020-02-12, 公開日: 2020-04-15, 最終更新日: 2024-06-19)

主引用文献

Sheppard, D.,Berry, J.L.,Denise, R.,Rocha, E.P.C.,Matthews, S.,Pelicic, V.
The major subunit of widespread competence pili exhibits a novel and conserved type IV pilin fold.
J.Biol.Chem., 295:6594-6604, 2020

PubMed Abstract: Type IV filaments (T4F), which are helical assemblies of type IV pilins, constitute a superfamily of filamentous nanomachines virtually ubiquitous in prokaryotes that mediate a wide variety of functions. The competence (Com) pilus is a widespread T4F, mediating DNA uptake (the first step in natural transformation) in bacteria with one membrane (monoderms), an important mechanism of horizontal gene transfer. Here, we report the results of genomic, phylogenetic, and structural analyses of ComGC, the major pilin subunit of Com pili. By performing a global comparative analysis, we show that Com pili genes are virtually ubiquitous in Bacilli, a major monoderm class of Firmicutes. This also revealed that ComGC displays extensive sequence conservation, defining a monophyletic group among type IV pilins. We further report ComGC solution structures from two naturally competent human pathogens, (ComGC) and (ComGC), revealing that this pilin displays extensive structural conservation. Strikingly, ComGC and ComGC exhibit a novel type IV pilin fold that is purely helical. Results from homology modeling analyses suggest that the unusual structure of ComGC is compatible with helical filament assembly. Because ComGC displays such a widespread distribution, these results have implications for hundreds of monoderm species.

PubMed: 32273343
DOI: 10.1074/jbc.RA120.013316

実験手法

SOLUTION NMR