6Y0D

Crystal structure of Trypanosoma cruzi antigen TcSMP11.90

6Y0D の概要

• エントリーDOI: 10.2210/pdb6y0d/pdb
• 分子名称: Surface membrane protein (2 entities in total)
• 機能のキーワード: trypanosoma cruzi, antigen, chagas, immune system
• 由来する生物種: Trypanosoma cruzi
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43406.46

構造登録者

Di Pisa, F.,Gourlay, L.J.,Bolognesi, M.,De Benedetti, S. (登録日: 2020-02-07, 公開日: 2022-02-02, 最終更新日: 2024-11-13)

主引用文献

Di Pisa, F.,De Benedetti, S.,Fassi, E.M.A.,Bombaci, M.,Grifantini, R.,Musico, A.,Frigerio, R.,Pontillo, A.,Rigo, C.,Abelli, S.,Grande, R.,Zanchetta, N.,Mileto, D.,Mancon, A.,Rizzo, A.,Gori, A.,Cretich, M.,Colombo, G.,Bolognesi, M.,Gourlay, L.J.
Elucidating the 3D Structure of a Surface Membrane Antigen from Trypanosoma cruzi as a Serodiagnostic Biomarker of Chagas Disease.
Vaccines (Basel), 10:-, 2022

PubMed Abstract: Chagas disease (CD) is a vector-borne parasitosis, caused by the protozoan parasite , that affects millions of people worldwide. Although endemic in South America, CD is emerging throughout the world due to climate change and increased immigratory flux of infected people to non-endemic regions. Containing of the diffusion of CD is challenged by the asymptomatic nature of the disease in early infection stages and by the lack of a rapid and effective diagnostic test. With the aim of designing new serodiagnostic molecules to be implemented in a microarray-based diagnostic set-up for early screening of CD, herein, we report the recombinant production of the extracellular domain of a surface membrane antigen from (SMP) and confirm its ability to detect plasma antibodies from infected patients. Moreover, we describe its high-resolution (1.62 Å) crystal structure, to which in silico epitope predictions were applied in order to locate the most immunoreactive regions of SMP in order to guide the design of epitopes that may be used as an alternative to the full-length antigen for CD diagnosis. Two putative, linear epitopes, belonging to the same immunogenic region, were synthesized as free peptides, and their immunological properties were tested in vitro. Although both peptides were shown to adopt a structural conformation that allowed their recognition by polyclonal antibodies raised against the recombinant protein, they were not serodiagnostic for infections. Nevertheless, they represent good starting points for further iterative structure-based (re)design cycles.

PubMed: 35062732
DOI: 10.3390/vaccines10010071

実験手法

X-RAY DIFFRACTION (1.62 Å)