6XY0

Crystal structure of a de novo designed parallel four-helix coiled coil, 3-EK-4

6XY0 の概要

• エントリーDOI: 10.2210/pdb6xy0/pdb
• 分子名称: 3-EK-4 (2 entities in total)
• 機能のキーワード: coiled coil, tetramer, parallel, de novo protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 13848.10

構造登録者

Edgell, C.L.,Savery, N.J.,Woolfson, D.N. (登録日: 2020-01-29, 公開日: 2020-03-18, 最終更新日: 2024-11-13)

主引用文献

Edgell, C.L.,Savery, N.J.,Woolfson, D.N.
RobustDe Novo-Designed Homotetrameric Coiled Coils.
Biochemistry, 59:1087-1092, 2020

PubMed Abstract: -designed protein domains are increasingly being applied in biotechnology, cell biology, and synthetic biology. Therefore, it is imperative that these proteins be robust to superficial changes; i.e., small changes to their amino acid sequences should not cause gross structural changes. In turn, this allows properties such as stability and solubility to be tuned without affecting structural attributes like tertiary fold and quaternary interactions. Reliably designed proteins with predictable behaviors may then be used as scaffolds to incorporate function, e.g., through the introduction of features for small-molecule, metal, or macromolecular binding, and enzyme-like active sites. Generally, achieving this requires the starting protein fold to be well understood. Herein, we focus on designing α-helical coiled coils, which are well studied, widespread, and often direct protein-protein interactions in natural systems. Our initial investigations reveal that a previously designed parallel, homotetrameric coiled coil, CC-Tet, is not robust to sequence changes that were anticipated to maintain its structure. Instead, the alterations switch the oligomeric state from tetramer to trimer. To improve the robustness of designed homotetramers, additional sequences based on CC-Tet were produced and characterized in solution and by X-ray crystallography. Of these updated sequences, one is robust to truncation and to changes in surface electrostatics; we call this CC-Tet*. Variants of the general CC-Tet* design provide a set of homotetrameric coiled coils with unfolding temperatures in the range from 40 to >95 °C. We anticipate that these will be of use in applications requiring robust and well-defined tetramerization domains.

PubMed: 32133841
DOI: 10.1021/acs.biochem.0c00082

実験手法

X-RAY DIFFRACTION (1.11 Å)