6XT6

pro-concanavalin A: Precursor of circularly permuted concanavalin A

6XT6 の概要

• エントリーDOI: 10.2210/pdb6xt6/pdb
• 分子名称: Concanavalin-A, MANGANESE (II) ION, CALCIUM ION, ... (6 entities in total)
• 機能のキーワード: lectin, carbohydrate-binding, plant protein
• 由来する生物種: Canavalia ensiformis (Jack bean)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 57816.59

構造登録者

Nonis, S.G.,Haywood, J.,Schmidberger, J.W.,Bond, C.S. (登録日: 2020-07-17, 公開日: 2020-11-11, 最終更新日: 2023-10-18)

主引用文献

Nonis, S.G.,Haywood, J.,Schmidberger, J.W.,Mackie, E.R.R.,Soares da Costa, T.P.,Bond, C.S.,Mylne, J.S.
Structural and biochemical analyses of concanavalin A circular permutation by jack bean asparaginyl endopeptidase.
Plant Cell, 33:2794-2811, 2021

PubMed Abstract: Over 30 years ago, an intriguing posttranslational modification was found responsible for creating concanavalin A (conA), a carbohydrate-binding protein from jack bean (Canavalia ensiformis) seeds and a common carbohydrate chromatography reagent. ConA biosynthesis involves what was then an unprecedented rearrangement in amino-acid sequence, whereby the N-terminal half of the gene-encoded conA precursor (pro-conA) is swapped to become the C-terminal half of conA. Asparaginyl endopeptidase (AEP) was shown to be involved, but its mechanism was not fully elucidated. To understand the structural basis and consequences of circular permutation, we generated recombinant jack bean pro-conA plus jack bean AEP (CeAEP1) and solved crystal structures for each to 2.1 and 2.7 Å, respectively. By reconstituting conA biosynthesis in vitro, we prove CeAEP1 alone can perform both cleavage and cleavage-coupled transpeptidation to form conA. CeAEP1 structural analysis reveals how it is capable of carrying out both reactions. Biophysical assays illustrated that pro-conA is less stable than conA. This observation was explained by fewer intermolecular interactions between subunits in the pro-conA crystal structure and consistent with a difference in the prevalence for tetramerization in solution. These findings elucidate the consequences of circular permutation in the only posttranslation example known to occur in nature.

PubMed: 34235541
DOI: 10.1093/plcell/koab130

実験手法

X-RAY DIFFRACTION (2.103 Å)