6XQH

Crystal structure of SCLam E144S mutant, a non-specific endo-beta-1,3(4)-glucanase from family GH16, co-crystallized with cellotriose, presenting a 1,3-beta-D-cellobiosyl-glucose and a cellobiose at active site

Summary for 6XQH

• Entry DOI: 10.2210/pdb6xqh/pdb
• Related: 6XOF
• Related PRD ID: PRD_900005
• Descriptor: GH16 family protein, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-3)-alpha-D-glucopyranose, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose, ... (5 entities in total)
• Functional Keywords: glycoside hydrolase, transglycosylation, endo-1, 3(4)-beta-glucanases, metagenome, hydrolase
• Biological source: uncultured bacterium
• Total number of polymer chains: 1
• Total formula weight: 31175.56

Authors

Liberato, M.V.,Squina, F. (deposition date: 2020-07-09, release date: 2021-02-10, Last modification date: 2023-10-18)

• Primary citation: Liberato, M.V.,Teixeira Prates, E.,Goncalves, T.A.,Bernardes, A.,Vilela, N.,Fattori, J.,Ematsu, G.C.,Chinaglia, M.,Machi Gomes, E.R.,Migliorini Figueira, A.C.,Damasio, A.,Polikarpov, I.,Skaf, M.S.,Squina, F.M.; Insights into the dual cleavage activity of the GH16 laminarinase enzyme class on beta-1,3 and beta-1,4 glycosidic bonds.; J.Biol.Chem., 296:100385-100385, 2021; PubMed: 33556371; DOI: 10.1016/j.jbc.2021.100385

Experimental method

X-RAY DIFFRACTION (1.57 Å)