6XMF

Cryo-EM structure of Cas12g binary complex

6XMF の概要

• エントリーDOI: 10.2210/pdb6xmf/pdb
• EMDBエントリー: 22257 22258
• 分子名称: CRISPR-Cas, RNA (116-MER), ZINC ION (3 entities in total)
• 機能のキーワード: crispr, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: metagenome; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 129313.51

構造登録者

Chang, L.,Li, Z.,Zhang, H. (登録日: 2020-06-30, 公開日: 2021-01-13, 最終更新日: 2025-05-28)

主引用文献

Li, Z.,Zhang, H.,Xiao, R.,Han, R.,Chang, L.
Cryo-EM structure of the RNA-guided ribonuclease Cas12g.
Nat.Chem.Biol., 17:387-393, 2021

PubMed Abstract: Cas12g, the type V-G CRISPR-Cas effector, is an RNA-guided ribonuclease that targets single-stranded RNA substrate. The CRISPR-Cas12g system offers a potential platform for transcriptome engineering and diagnostic applications. We determined the structures of Cas12g-guide RNA complexes in the absence and presence of target RNA by cryo-EM to a resolution of 3.1 Å and 4.8 Å, respectively. Cas12g adopts a bilobed structure with miniature REC2 and Nuc domains, whereas the guide RNAs fold into a flipped 'F' shape, which is primarily recognized by the REC lobe. Target RNA and the CRISPR RNA (crRNA) guide form a duplex that inserts into the central cavity between the REC and NUC lobes, inducing conformational changes in both lobes to activate Cas12g. The structural insights would facilitate the development of Cas12g-based applications.

PubMed: 33495647
DOI: 10.1038/s41589-020-00721-2

実験手法

ELECTRON MICROSCOPY (3.1 Å)