6XJV

MCU holocomplex in High-calcium state

6XJV の概要

• エントリーDOI: 10.2210/pdb6xjv/pdb
• EMDBエントリー: 22215
• 分子名称: Calcium uniporter protein, mitochondrial, Essential MCU regulator, mitochondrial, Calcium uptake protein 1, mitochondrial, ... (4 entities in total)
• 機能のキーワード: ion channel, transport protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 619348.54

構造登録者

Wang, Y.,Jiang, Y. (登録日: 2020-06-24, 公開日: 2020-09-09, 最終更新日: 2024-03-06)

主引用文献

Wang, Y.,Han, Y.,She, J.,Nguyen, N.X.,Mootha, V.K.,Bai, X.C.,Jiang, Y.
Structural insights into the Ca 2+ -dependent gating of the human mitochondrial calcium uniporter.
Elife, 9:-, 2020

PubMed Abstract: Mitochondrial Ca uptake is mediated by an inner mitochondrial membrane protein called the mitochondrial calcium uniporter. In humans, the uniporter functions as a holocomplex consisting of MCU, EMRE, MICU1 and MICU2, among which MCU and EMRE form a subcomplex and function as the conductive channel while MICU1 and MICU2 are EF-hand proteins that regulate the channel activity in a Ca-dependent manner. Here, we present the EM structures of the human mitochondrial calcium uniporter holocomplex (uniplex) in the presence and absence of Ca, revealing distinct Ca dependent assembly of the uniplex. Our structural observations suggest that Ca changes the dimerization interaction between MICU1 and MICU2, which in turn determines how the MICU1-MICU2 subcomplex interacts with the MCU-EMRE channel and, consequently, changes the distribution of the uniplex assemblies between the blocked and unblocked states.

PubMed: 32762847
DOI: 10.7554/eLife.60513

実験手法

ELECTRON MICROSCOPY (4.17 Å)