6XJM

Biuret Hydrolase (BiuH) from Rhodococcus sp. Mel C169S bound with biuret

6XJM の概要

• エントリーDOI: 10.2210/pdb6xjm/pdb
• 関連するPDBエントリー: 6XIX 6XJ4 6XJE
• 分子名称: Biuret hydrolase, dicarbonimidic diamide (3 entities in total)
• 機能のキーワード: trta, biuh, triuret, biuret, cysteine hydrolase, nitrogen, hydrolase
• 由来する生物種: Rhodococcus sp. Mel
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 104589.66

構造登録者

Tassoulas, L.T.,Elias, M.H.,Wackett, L.P. (登録日: 2020-06-24, 公開日: 2020-11-18, 最終更新日: 2023-10-18)

主引用文献

Tassoulas, L.J.,Elias, M.H.,Wackett, L.P.
Discovery of an ultraspecific triuret hydrolase (TrtA) establishes the triuret biodegradation pathway.
J.Biol.Chem., 296:100055-100055, 2020

PubMed Abstract: Triuret (carbonyldiurea) is an impurity found in industrial urea fertilizer (<0.1% w/w) that is applied, worldwide, around 300 million pounds each year on agricultural lands. In addition to anthropogenic sources, endogenous triuret has been identified in amoeba and human urine, the latter being diagnostic for hypokalemia. The present study is the first to describe the metabolic breakdown of triuret, which funnels into biuret metabolism. We identified the gene responsible for triuret decomposition (trtA) in bacterial genomes, clustered with biuH, which encodes biuret hydrolase and has close protein sequence homology. TrtA is a member of the isochorismatase-like hydrolase (IHL) protein family, similarly to BiuH, and has a catalytic efficiency (kK) of 6 x 10 Ms, a K for triuret of 20 μM, and exquisite substrate specificity. Indeed, TrtA has four orders of magnitude less activity with biuret. Crystal structures of TrtA in apo and holo form were solved and compared with the BiuH structure. The high substrate selectivity was found to be conveyed by second shell residues around each active site. Mutagenesis of residues conserved in TrtA to the alternate consensus found in BiuHs revealed residues critical to triuret hydrolase activity but no single mutant evolved more biuret activity, and likely a combination of mutations is required to interconvert between TrtA, BiuH functions. TrtA-mediated triuret metabolism is relatively rare in recorded genomes (1-2%), but is largely found in plant-associated, nodulating, and endophytic bacteria. This study suggests functions for triuret hydrolase in certain eukaryotic intermediary processes and prokaryotic intermediary or biodegradative metabolism.

PubMed: 33172891
DOI: 10.1074/jbc.RA120.015631

実験手法

X-RAY DIFFRACTION (2.05 Å)