6XJI

PmtCD ABC exporter at C1 symmetry

6XJI の概要

• エントリーDOI: 10.2210/pdb6xji/pdb
• 関連するPDBエントリー: 6U2D 6XFU 6XJH
• EMDBエントリー: 20640 22194 22210 22211
• 分子名称: Phenol-soluble modulin export ABC transporter permease subunit PmtD, ABC transporter ATP-binding protein, PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER, ... (4 entities in total)
• 機能のキーワード: abc transporter, abc exporter, peptide transort, membrane protein
• 由来する生物種: Staphylococcus aureus; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 128074.06

構造登録者

Zeytuni, N.,Strynadka, N.J.C.,Hu, J.,Worrall, L.J.,Chou, H.,Yu, Z. (登録日: 2020-06-23, 公開日: 2020-10-14, 最終更新日: 2025-05-28)

主引用文献

Zeytuni, N.,Dickey, S.W.,Hu, J.,Chou, H.T.,Worrall, L.J.,Alexander, J.A.N.,Carlson, M.L.,Nosella, M.,Duong, F.,Yu, Z.,Otto, M.,Strynadka, N.C.J.
Structural insight into the Staphylococcus aureus ATP-driven exporter of virulent peptide toxins
Sci Adv, 6:eabb8219-, 2020

PubMed Abstract: is a major human pathogen that has acquired alarming broad-spectrum antibiotic resistance. One group of secreted toxins with key roles during infection is the phenol-soluble modulins (PSMs). PSMs are amphipathic, membrane-destructive cytolytic peptides that are exported to the host-cell environment by a designated adenosine 5'-triphosphate (ATP)-binding cassette (ABC) transporter, the PSM transporter (PmtABCD). Here, we demonstrate that the minimal Pmt unit necessary for PSM export is PmtCD and provide its first atomic characterization by single-particle cryo-EM and x-ray crystallography. We have captured the transporter in the ATP-bound state at near atomic resolution, revealing a type II ABC exporter fold, with an additional cytosolic domain. Comparison to a lower-resolution nucleotide-free map displaying an "open" conformation and putative hydrophobic inner chamber of a size able to accommodate the binding of two PSM peptides provides mechanistic insight and sets the foundation for therapeutic design.

PubMed: 32998902
DOI: 10.1126/sciadv.abb8219

実験手法

ELECTRON MICROSCOPY (4 Å)