6XE1

Structure of SARS-CoV-2 spike protein receptor binding domain in complex with a potent neutralizing antibody, CV30 Fab

6XE1 の概要

• エントリーDOI: 10.2210/pdb6xe1/pdb
• 分子名称: CV30 Fab Heavy chain, CV30 Fab Kappa chain, Spike protein S1, ... (5 entities in total)
• 機能のキーワード: antibody, covid-19, immune system, viral protein, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 77691.76

構造登録者

Hurlburt, N.K.,Pancera, M. (登録日: 2020-06-11, 公開日: 2020-07-01, 最終更新日: 2024-10-09)

主引用文献

Hurlburt, N.K.,Seydoux, E.,Wan, Y.H.,Edara, V.V.,Stuart, A.B.,Feng, J.,Suthar, M.S.,McGuire, A.T.,Stamatatos, L.,Pancera, M.
Structural basis for potent neutralization of SARS-CoV-2 and role of antibody affinity maturation.
Nat Commun, 11:5413-5413, 2020

PubMed Abstract: SARS-CoV-2 is a betacoronavirus virus responsible for the COVID-19 pandemic. Here, we determine the X-ray crystal structure of a potent neutralizing monoclonal antibody, CV30, isolated from a patient infected with SARS-CoV-2, in complex with the receptor binding domain. The structure reveals that CV30 binds to an epitope that overlaps with the human ACE2 receptor binding motif providing a structural basis for its neutralization. CV30 also induces shedding of the S1 subunit, indicating an additional mechanism of neutralization. A germline reversion of CV30 results in a substantial reduction in both binding affinity and neutralization potential indicating the minimal somatic mutation is needed for potently neutralizing antibodies against SARS-CoV-2.

PubMed: 33110068
DOI: 10.1038/s41467-020-19231-9

実験手法

X-RAY DIFFRACTION (2.75 Å)