6X66

Legionella pneumophila dDot T4SS OMC

これはPDB形式変換不可エントリーです。

6X66 の概要

• エントリーDOI: 10.2210/pdb6x66/pdb
• EMDBエントリー: 22071
• 分子名称: DotC, DotD, Type IV secretion protein IcmK, ... (6 entities in total)
• 機能のキーワード: secretion, t4ss, dot, protein transport
• 由来する生物種: Legionella pneumophila; 詳細
• タンパク質・核酸の鎖数: 117
• 化学式量合計: 3143773.59

構造登録者

Durie, C.L.,Sheedlo, M.J.,Chung, J.M.,Byrne, B.G.,Su, M.,Knight, T.,Swanson, M.S.,Lacy, D.B.,Ohi, M.D. (登録日: 2020-05-27, 公開日: 2020-10-28, 最終更新日: 2024-03-06)

主引用文献

Durie, C.L.,Sheedlo, M.J.,Chung, J.M.,Byrne, B.G.,Su, M.,Knight, T.,Swanson, M.,Lacy, D.B.,Ohi, M.D.
Structural analysis of the Legionella pneumophila Dot/Icm type IV secretion system core complex.
Elife, 9:-, 2020

PubMed Abstract: is an opportunistic pathogen that causes the potentially fatal pneumonia Legionnaires' Disease. This infection and subsequent pathology require the Dot/Icm Type IV Secretion System (T4SS) to deliver effector proteins into host cells. Compared to prototypical T4SSs, the Dot/Icm assembly is much larger, containing ~27 different components including a core complex reported to be composed of five proteins: DotC, DotD, DotF, DotG, and DotH. Using single particle cryo-electron microscopy (cryo-EM), we report reconstructions of the core complex of the Dot/Icm T4SS that includes a symmetry mismatch between distinct structural features of the outer membrane cap (OMC) and periplasmic ring (PR). We present models of known core complex proteins, DotC, DotD, and DotH, and two structurally similar proteins within the core complex, DotK and Lpg0657. This analysis reveals the stoichiometry and contact interfaces between the key proteins of the Dot/Icm T4SS core complex and provides a framework for understanding a complex molecular machine.

PubMed: 32876045
DOI: 10.7554/eLife.59530

実験手法

ELECTRON MICROSCOPY (4.2 Å)