Summary for 6X5I
| Entry DOI | 10.2210/pdb6x5i/pdb |
| EMDB information | 22051 |
| Descriptor | 1-KMe3 peptide-like fibril (1 entity in total) |
| Functional Keywords | peptide-like fibril, helical symmetry, protein fibril |
| Biological source | Homo sapiens |
| Total number of polymer chains | 70 |
| Total formula weight | 61667.55 |
| Authors | Wang, F.,Feng, Z.,Xu, B.,Egelman, E.H. (deposition date: 2020-05-26, release date: 2020-06-03, Last modification date: 2020-08-26) |
| Primary citation | Feng, Z.,Wang, H.,Wang, F.,Oh, Y.,Berciu, C.,Cui, Q.,Egelman, E.H.,Xu, B. Artificial Intracellular Filaments. Cell Rep Phys Sci, 1:-, 2020 Cited by PubMed Abstract: Intracellular protein filaments are ubiquitous for cellular functions, but forming bona fide biomimetic intracellular filaments of small molecules in living cells remains elusive. Here, we report the formation of self-limiting intracellular filaments of a small peptide via enzymatic morphological transition of a phosphorylated and trimethylated heterochiral tetrapeptide. Enzymatic dephosphorylation reduces repulsive intermolecular electrostatic interactions and converts the peptidic nanoparticles into filaments, which exhibit distinct types of cross-β structures with either C7 or C2 symmetries, with the hydrophilic C-terminal residues at the periphery of the helix. Macromolecular crowding promotes the peptide filaments to form bundles, which extend from the plasma membrane to nuclear membrane and hardly interact with endogenous components, including cytoskeletons. Stereochemistry and post-translational modification (PTM) of peptides are critical for generating the intracellular bundles. This work may offer a way to gain lost functions or to provide molecular insights for understanding normal and aberrant intracellular filaments. PubMed: 32776017DOI: 10.1016/j.xcrp.2020.100085 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.3 Å) |
Structure validation
Download full validation report
