6WYI

Crystal structure of EchA19, enoyl-CoA hydratase from Mycobacterium tuberculosis

6WYI の概要

• エントリーDOI: 10.2210/pdb6wyi/pdb
• 分子名称: EchA19, enoyl-CoA hydratase, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: mycobacterium tuberculosis, cholesterol metabolism, enoyl-coa hydratase, lyase
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30578.35

構造登録者

Bonds, A.C.,Garcia-Diaz, M.,Sampson, N.S. (登録日: 2020-05-13, 公開日: 2020-07-29, 最終更新日: 2023-10-18)

主引用文献

Bonds, A.C.,Yuan, T.,Werman, J.M.,Jang, J.,Lu, R.,Nesbitt, N.M.,Garcia-Diaz, M.,Sampson, N.S.
Post-translational Succinylation ofMycobacterium tuberculosisEnoyl-CoA Hydratase EchA19 Slows Catalytic Hydration of Cholesterol Catabolite 3-Oxo-chol-4,22-diene-24-oyl-CoA.
Acs Infect Dis., 6:2214-2224, 2020

PubMed Abstract: Cholesterol is a major carbon source for () during infection, and cholesterol utilization plays a significant role in persistence and virulence within host macrophages. Elucidating the mechanism by which cholesterol is degraded may permit the identification of new therapeutic targets. Here, we characterized EchA19 (Rv3516), an enoyl-CoA hydratase involved in cholesterol side-chain catabolism. Steady-state kinetics assays demonstrated that EchA19 preferentially hydrates cholesterol enoyl-CoA metabolite 3-oxo-chol-4,22-diene-24-oyl-CoA, an intermediate of side-chain β-oxidation. In addition, succinyl-CoA, a downstream catabolite of propionyl-CoA that forms during cholesterol degradation, covalently modifies targeted mycobacterial proteins, including EchA19. Inspection of a 1.9 Å resolution X-ray crystallography structure of EchA19 suggests that succinylation of Lys132 and Lys139 may perturb enzymatic activity by modifying the entrance to the substrate binding site. Treatment of EchA19 with succinyl-CoA revealed that these two residues are hotspots for succinylation. Replacement of these specific lysine residues with negatively charged glutamate reduced the rate of catalytic hydration of 3-oxo-chol-4,22-diene-24-oyl-CoA by EchA19, as does succinylation of EchA19. Our findings suggest that succinylation is a negative feedback regulator of cholesterol metabolism, thereby adding another layer of complexity to physiology in the host. These regulatory pathways are potential noncatabolic targets for antimicrobial drugs.

PubMed: 32649175
DOI: 10.1021/acsinfecdis.0c00329

実験手法

X-RAY DIFFRACTION (1.915 Å)