6WUE
Tetragonal crystal form of SbtB from Synechocystis PCC6803
Summary for 6WUE
| Entry DOI | 10.2210/pdb6wue/pdb |
| Descriptor | Membrane-associated protein slr1513, ISOPROPYL ALCOHOL, SODIUM ION, ... (4 entities in total) |
| Functional Keywords | regulator of cyanobacterial bicarbonate transporter, signaling protein |
| Biological source | Synechocystis sp. |
| Total number of polymer chains | 2 |
| Total formula weight | 24186.80 |
| Authors | Bu, G.,Simmons, C.R.,Nielsen, D.R.,Nannenga, B.L. (deposition date: 2020-05-04, release date: 2020-08-26, Last modification date: 2024-10-23) |
| Primary citation | Bu, G.,Simmons, C.R.,Nielsen, D.R.,Nannenga, B.L. Tetragonal crystal form of the cyanobacterial bicarbonate-transporter regulator SbtB from Synechocystis sp. PCC 6803. Acta Crystallogr.,Sect.F, 76:438-443, 2020 Cited by PubMed Abstract: The P-like protein SbtB has been identified as a regulator of SbtA, which is one of the key bicarbonate transporters in cyanobacteria. While SbtB from Synechocystis sp. PCC 6803 has previously been shown to be a trimer, a new crystal form is reported here which crystallizes in what is thought to be a non-native tetramer in the crystal, with the C-terminus in an extended conformation. The crystal structure shows the formation of an intermolecular disulfide bond at Cys94 between SbtB monomers, which may stabilize this conformation in the crystal. This motivates the need for future studies to investigate the potential role that the oxidation and reduction of these cysteines may play in the activation and/or function of SbtB. PubMed: 32880592DOI: 10.1107/S2053230X20010523 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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