6WUE

Tetragonal crystal form of SbtB from Synechocystis PCC6803

6WUE の概要

• エントリーDOI: 10.2210/pdb6wue/pdb
• 分子名称: Membrane-associated protein slr1513, ISOPROPYL ALCOHOL, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: regulator of cyanobacterial bicarbonate transporter, signaling protein
• 由来する生物種: Synechocystis sp.
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 24186.80

構造登録者

Bu, G.,Simmons, C.R.,Nielsen, D.R.,Nannenga, B.L. (登録日: 2020-05-04, 公開日: 2020-08-26, 最終更新日: 2024-10-23)

主引用文献

Bu, G.,Simmons, C.R.,Nielsen, D.R.,Nannenga, B.L.
Tetragonal crystal form of the cyanobacterial bicarbonate-transporter regulator SbtB from Synechocystis sp. PCC 6803.
Acta Crystallogr.,Sect.F, 76:438-443, 2020

PubMed Abstract: The P-like protein SbtB has been identified as a regulator of SbtA, which is one of the key bicarbonate transporters in cyanobacteria. While SbtB from Synechocystis sp. PCC 6803 has previously been shown to be a trimer, a new crystal form is reported here which crystallizes in what is thought to be a non-native tetramer in the crystal, with the C-terminus in an extended conformation. The crystal structure shows the formation of an intermolecular disulfide bond at Cys94 between SbtB monomers, which may stabilize this conformation in the crystal. This motivates the need for future studies to investigate the potential role that the oxidation and reduction of these cysteines may play in the activation and/or function of SbtB.

PubMed: 32880592
DOI: 10.1107/S2053230X20010523

実験手法

X-RAY DIFFRACTION (1.8 Å)