6WUC

The yeast Ctf3 complex with Cnn1-Wip1

6WUC の概要

• エントリーDOI: 10.2210/pdb6wuc/pdb
• EMDBエントリー: 0523 20200 21910
• 分子名称: Inner kinetochore subunit MCM16, Inner kinetochore subunit CTF3, Inner kinetochore subunit MCM22, ... (5 entities in total)
• 機能のキーワード: kinetochore, cell cycle, chromosome segregation, ccan
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 185818.55

構造登録者

Hinshaw, S.M.,Harrison, S.C. (登録日: 2020-05-04, 公開日: 2020-06-17, 最終更新日: 2025-06-04)

主引用文献

Hinshaw, S.M.,Harrison, S.C.
The Structural Basis for Kinetochore Stabilization by Cnn1/CENP-T.
Curr.Biol., 30:3425-3431.e3, 2020

PubMed Abstract: Chromosome segregation depends on a regulated connection between spindle microtubules and centromeric DNA. The kinetochore mediates this connection and ensures it persists during anaphase, when sister chromatids must transit into daughter cells uninterrupted. The Ctf19 complex (Ctf19c) forms the centromeric base of the kinetochore in budding yeast. Biochemical experiments show that Ctf19c members associate hierarchically when purified from cell extract [1], an observation that is mostly explained by the structure of the complex [2]. The Ctf3 complex (Ctf3c), which is not required for the assembly of most other Ctf19c factors, disobeys the biochemical assembly hierarchy when observed in dividing cells that lack more basal components [3]. Thus, the biochemical experiments do not completely recapitulate the logic of centromeric Ctf19c assembly. We now present a high-resolution structure of the Ctf3c bound to the Cnn1-Wip1 heterodimer. Associated live-cell imaging experiments provide a mechanism for Ctf3c and Cnn1-Wip1 recruitment to the kinetochore. The mechanism suggests feedback regulation of Ctf19c assembly and unanticipated similarities in kinetochore organization between yeast and vertebrates.

PubMed: 32679099
DOI: 10.1016/j.cub.2020.06.024

実験手法

ELECTRON MICROSCOPY (3.23 Å)