6WU9

50S subunit of 70S Ribosome Enterococcus faecalis MultiBody refinement

6WU9 の概要

• エントリーDOI: 10.2210/pdb6wu9/pdb
• EMDBエントリー: 0656 0657 0658 0659 0660 21907
• 分子名称: 23S rRNA, 50S ribosomal protein L16, 50S ribosomal protein L17, ... (28 entities in total)
• 機能のキーワード: 70s, ribosome, pathogen, antibiotic development, antibiotic resistant
• 由来する生物種: Enterococcus faecalis OG1RF; 詳細
• タンパク質・核酸の鎖数: 27
• 化学式量合計: 1280360.81

構造登録者

Jogl, G.,Khayat, R. (登録日: 2020-05-04, 公開日: 2020-11-04, 最終更新日: 2024-03-06)

主引用文献

Murphy, E.L.,Singh, K.V.,Avila, B.,Kleffmann, T.,Gregory, S.T.,Murray, B.E.,Krause, K.L.,Khayat, R.,Jogl, G.
Cryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis.
Sci Rep, 10:16301-16301, 2020

PubMed Abstract: Enterococcus faecalis is a gram-positive organism responsible for serious infections in humans, but as with many bacterial pathogens, resistance has rendered a number of commonly used antibiotics ineffective. Here, we report the cryo-EM structure of the E. faecalis 70S ribosome to a global resolution of 2.8 Å. Structural differences are clustered in peripheral and solvent exposed regions when compared with Escherichia coli, whereas functional centres, including antibiotic binding sites, are similar to other bacterial ribosomes. Comparison of intersubunit conformations among five classes obtained after three-dimensional classification identifies several rotated states. Large ribosomal subunit protein bL31, which forms intersubunit bridges to the small ribosomal subunit, assumes different conformations in the five classes, revealing how contacts to the small subunit are maintained throughout intersubunit rotation. A tRNA observed in one of the five classes is positioned in a chimeric pe/E position in a rotated ribosomal state. The 70S ribosome structure of E. faecalis now extends our knowledge of bacterial ribosome structures and may serve as a basis for the development of novel antibiotic compounds effective against this pathogen.

PubMed: 33004869
DOI: 10.1038/s41598-020-73199-6

実験手法

ELECTRON MICROSCOPY (2.9 Å)