6WT9

Structure of STING-associated CdnE c-di-GMP synthase from Capnocytophaga granulosa

Summary for 6WT9

• Entry DOI: 10.2210/pdb6wt9/pdb
• Descriptor: NTP_transf_2 domain-containing protein (2 entities in total)
• Functional Keywords: cbass, cd-ntase, c-di-gmp, sting, transferase
• Biological source: Capnocytophaga granulosa
• Total number of polymer chains: 1
• Total formula weight: 41378.11

Authors

Morehouse, B.R.,Govande, A.A.,Millman, A.,Keszei, A.F.A.,Lowey, B.,Ofir, G.,Shao, S.,Sorek, R.,Kranzusch, P.J. (deposition date: 2020-05-01, release date: 2020-09-09, Last modification date: 2020-10-28)

Primary citation

Morehouse, B.R.,Govande, A.A.,Millman, A.,Keszei, A.F.A.,Lowey, B.,Ofir, G.,Shao, S.,Sorek, R.,Kranzusch, P.J.
STING cyclic dinucleotide sensing originated in bacteria.
Nature, 586:429-433, 2020

PubMed Abstract: Stimulator of interferon genes (STING) is a receptor in human cells that senses foreign cyclic dinucleotides that are released during bacterial infection and in endogenous cyclic GMP-AMP signalling during viral infection and anti-tumour immunity. STING shares no structural homology with other known signalling proteins, which has limited attempts at functional analysis and prevented explanation of the origin of cyclic dinucleotide signalling in mammalian innate immunity. Here we reveal functional STING homologues encoded within prokaryotic defence islands, as well as a conserved mechanism of signal activation. Crystal structures of bacterial STING define a minimal homodimeric scaffold that selectively responds to cyclic di-GMP synthesized by a neighbouring cGAS/DncV-like nucleotidyltransferase (CD-NTase) enzyme. Bacterial STING domains couple the recognition of cyclic dinucleotides with the formation of protein filaments to drive oligomerization of TIR effector domains and rapid NAD cleavage. We reconstruct the evolutionary events that followed the acquisition of STING into metazoan innate immunity, and determine the structure of a full-length TIR-STING fusion from the Pacific oyster Crassostrea gigas. Comparative structural analysis demonstrates how metazoan-specific additions to the core STING scaffold enabled a switch from direct effector function to regulation of antiviral transcription. Together, our results explain the mechanism of STING-dependent signalling and reveal the conservation of a functional cGAS-STING pathway in prokaryotic defence against bacteriophages.

PubMed: 32877915
DOI: 10.1038/s41586-020-2719-5

Experimental method

X-RAY DIFFRACTION (2.3 Å)