6WM9
Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to human monoclonal antibody 237235
Summary for 6WM9
| Entry DOI | 10.2210/pdb6wm9/pdb |
| Descriptor | Reticulocyte binding protein 2b, 237235 Fab heavy chain, 237235 Fab light chain, ... (4 entities in total) |
| Functional Keywords | invasion, plasmodium vivax, malaria, antibody complex, cell invasion |
| Biological source | Plasmodium vivax (strain Salvador I) More |
| Total number of polymer chains | 6 |
| Total formula weight | 170513.79 |
| Authors | Chan, L.J.,Dietrich, M.H.,Tham, W.H. (deposition date: 2020-04-20, release date: 2021-01-27, Last modification date: 2024-10-09) |
| Primary citation | Chan, L.J.,Gandhirajan, A.,Carias, L.L.,Dietrich, M.H.,Vadas, O.,Visentin, R.,Franca, C.T.,Menant, S.,Soldati-Favre, D.,Mueller, I.,King, C.L.,Tham, W.H. Naturally acquired blocking human monoclonal antibodies to Plasmodium vivax reticulocyte binding protein 2b. Nat Commun, 12:1538-1538, 2021 Cited by PubMed Abstract: Plasmodium vivax preferentially invades reticulocytes and recognition of these cells is mediated by P. vivax Reticulocyte Binding Protein 2b (PvRBP2b) binding to human Transferrin receptor 1 (TfR1) and Transferrin (Tf). Longitudinal cohort studies in Papua New Guinea, Thailand and Brazil show that PvRBP2b antibodies are correlated with protection against P. vivax infection and disease. Here, we isolate and characterize anti-PvRBP2b human monoclonal antibodies from two individuals in Cambodia with natural P. vivax infection. These antibodies bind with high affinities and map to different regions of PvRBP2b. Several human antibodies block PvRBP2b binding to reticulocytes and inhibit complex formation with human TfR1-Tf. We describe different structural mechanisms for functional inhibition, including either steric hindrance with TfR1-Tf or the reticulocyte membrane. These results show that naturally acquired human antibodies against PvRBP2b can inhibit its function which is important for P. vivax invasion. PubMed: 33750786DOI: 10.1038/s41467-021-21811-2 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.45 Å) |
Structure validation
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