6WLO

hc16 ligase models, 11.0 Angstrom resolution

6WLO の概要

• エントリーDOI: 10.2210/pdb6wlo/pdb
• 関連するPDBエントリー: 6WLN
• EMDBエントリー: 21831 21832 21833 21834 21835 21836 21838 21839 21840 21841 21842
• 分子名称: RNA (338-MER) (1 entity in total)
• 機能のキーワード: ribozyme, rna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 109298.71

構造登録者

Kappel, K.,Zhang, K.,Su, Z.,Watkins, A.M.,Kladwang, W.,Li, S.,Pintilie, G.,Topkar, V.V.,Rangan, R.,Zheludev, I.N.,Yesselman, J.D.,Chiu, W.,Das, R. (登録日: 2020-04-20, 公開日: 2020-07-08, 最終更新日: 2024-03-06)

主引用文献

Kappel, K.,Zhang, K.,Su, Z.,Watkins, A.M.,Kladwang, W.,Li, S.,Pintilie, G.,Topkar, V.V.,Rangan, R.,Zheludev, I.N.,Yesselman, J.D.,Chiu, W.,Das, R.
Accelerated cryo-EM-guided determination of three-dimensional RNA-only structures.
Nat.Methods, 17:699-707, 2020

PubMed Abstract: The discovery and design of biologically important RNA molecules is outpacing three-dimensional structural characterization. Here, we demonstrate that cryo-electron microscopy can routinely resolve maps of RNA-only systems and that these maps enable subnanometer-resolution coordinate estimation when complemented with multidimensional chemical mapping and Rosetta DRRAFTER computational modeling. This hybrid 'Ribosolve' pipeline detects and falsifies homologies and conformational rearrangements in 11 previously unknown 119- to 338-nucleotide protein-free RNA structures: full-length Tetrahymena ribozyme, hc16 ligase with and without substrate, full-length Vibrio cholerae and Fusobacterium nucleatum glycine riboswitch aptamers with and without glycine, Mycobacterium SAM-IV riboswitch with and without S-adenosylmethionine, and the computer-designed ATP-TTR-3 aptamer with and without AMP. Simulation benchmarks, blind challenges, compensatory mutagenesis, cross-RNA homologies and internal controls demonstrate that Ribosolve can accurately resolve the global architectures of RNA molecules but does not resolve atomic details. These tests offer guidelines for making inferences in future RNA structural studies with similarly accelerated throughput.

PubMed: 32616928
DOI: 10.1038/s41592-020-0878-9

実験手法

ELECTRON MICROSCOPY (11 Å)