6WK8

Crystal structure of Gdx-Clo from Small Multidrug Resistance family of transporters in complex with phenylguanidinium

6WK8 の概要

• エントリーDOI: 10.2210/pdb6wk8/pdb
• 分子名称: Multidrug resistance protein, SMR family, L10 monobody, 1-phenylguanidine, ... (5 entities in total)
• 機能のキーワード: small multidrug resistance, guanidinium transporter, emre homologue, dual topology protein, transport protein
• 由来する生物種: Clostridiales bacterium oral taxon 876 str. F0540; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 43411.69

構造登録者

Kermani, A.A.,Stockbridge, R.B. (登録日: 2020-04-15, 公開日: 2020-10-28, 最終更新日: 2024-03-06)

主引用文献

Kermani, A.A.,Macdonald, C.B.,Burata, O.E.,Ben Koff, B.,Koide, A.,Denbaum, E.,Koide, S.,Stockbridge, R.B.
The structural basis of promiscuity in small multidrug resistance transporters.
Nat Commun, 11:6064-6064, 2020

PubMed Abstract: By providing broad resistance to environmental biocides, transporters from the small multidrug resistance (SMR) family drive the spread of multidrug resistance cassettes among bacterial populations. A fundamental understanding of substrate selectivity by SMR transporters is needed to identify the types of selective pressures that contribute to this process. Using solid-supported membrane electrophysiology, we find that promiscuous transport of hydrophobic substituted cations is a general feature of SMR transporters. To understand the molecular basis for promiscuity, we solved X-ray crystal structures of a SMR transporter Gdx-Clo in complex with substrates to a maximum resolution of 2.3 Å. These structures confirm the family's extremely rare dual topology architecture and reveal a cleft between two helices that provides accommodation in the membrane for the hydrophobic substituents of transported drug-like cations.

PubMed: 33247110
DOI: 10.1038/s41467-020-19820-8

実験手法

X-RAY DIFFRACTION (2.53 Å)