6WK3
Engineered carbene transferase RmaNOD Q52V, putative nitric oxide dioxygenase from Rhodothermus marinus
Summary for 6WK3
| Entry DOI | 10.2210/pdb6wk3/pdb |
| Descriptor | Nitric oxide dioxygenase, PROTOPORPHYRIN IX CONTAINING FE, ACETATE ION, ... (5 entities in total) |
| Functional Keywords | heme binding protein, carbene transferase, globin, metal binding protein |
| Biological source | Rhodothermus marinus DSM 4252 |
| Total number of polymer chains | 4 |
| Total formula weight | 68710.16 |
| Authors | Knight, A.M.,Arnold, F.H. (deposition date: 2020-04-15, release date: 2020-07-08, Last modification date: 2024-03-06) |
| Primary citation | Wittmann, B.J.,Knight, A.M.,Hofstra, J.L.,Reisman, S.E.,Kan, S.B.J.,Arnold, F.H. Diversity-Oriented Enzymatic Synthesis of Cyclopropane Building Blocks. Acs Catalysis, 10:7112-7116, 2020 Cited by PubMed Abstract: While biocatalysis is increasingly incorporated into drug development pipelines, it is less commonly used in the early stages of drug discovery. By engineering a protein to produce a chiral motif with a derivatizable functional handle, biocatalysts can be used to help generate diverse building blocks for drug discovery. Here we show the engineering of two variants of nitric oxide dioxygenase (NOD) to catalyze the formation of - and diastereomers of a pinacolboronate-substituted cyclopropane which can be readily derivatized to generate diverse stereopure cyclopropane building blocks. PubMed: 33282460DOI: 10.1021/acscatal.0c01888 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.45 Å) |
Structure validation
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