6WI5

De novo designed protein Foldit4

6WI5 の概要

• エントリーDOI: 10.2210/pdb6wi5/pdb
• 関連するPDBエントリー: 6MRR 6MRS
• 分子名称: De novo designed protein Foldit4 (2 entities in total)
• 機能のキーワード: foldit, de novo design, de novo protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22063.48

構造登録者

Bera, A.K.,Koepnick, B.,Boykov, A.,Baker, D. (登録日: 2020-04-08, 公開日: 2020-04-22, 最終更新日: 2024-04-03)

主引用文献

Chen, Z.,Kibler, R.D.,Hunt, A.,Busch, F.,Pearl, J.,Jia, M.,VanAernum, Z.L.,Wicky, B.I.M.,Dods, G.,Liao, H.,Wilken, M.S.,Ciarlo, C.,Green, S.,El-Samad, H.,Stamatoyannopoulos, J.,Wysocki, V.H.,Jewett, M.C.,Boyken, S.E.,Baker, D.
De novo design of protein logic gates.
Science, 368:78-84, 2020

PubMed Abstract: The design of modular protein logic for regulating protein function at the posttranscriptional level is a challenge for synthetic biology. Here, we describe the design of two-input AND, OR, NAND, NOR, XNOR, and NOT gates built from de novo-designed proteins. These gates regulate the association of arbitrary protein units ranging from split enzymes to transcriptional machinery in vitro, in yeast and in primary human T cells, where they control the expression of the gene related to T cell exhaustion. Designed binding interaction cooperativity, confirmed by native mass spectrometry, makes the gates largely insensitive to stoichiometric imbalances in the inputs, and the modularity of the approach enables ready extension to three-input OR, AND, and disjunctive normal form gates. The modularity and cooperativity of the control elements, coupled with the ability to de novo design an essentially unlimited number of protein components, should enable the design of sophisticated posttranslational control logic over a wide range of biological functions.

PubMed: 32241946
DOI: 10.1126/science.aay2790

実験手法

X-RAY DIFFRACTION (1.83 Å)