6WEY

High-resolution structure of the SARS-CoV-2 NSP3 Macro X domain

6WEY の概要

• エントリーDOI: 10.2210/pdb6wey/pdb
• 関連するPDBエントリー: 6VXS
• 分子名称: Non-structural protein 3 (2 entities in total)
• 機能のキーワード: macro domain, adp-ribose-binding, sars-cov-2, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2, COVID-19 virus)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18494.11

構造登録者

Vuksanovic, N.,Silvaggi, N.R. (登録日: 2020-04-03, 公開日: 2020-04-29, 最終更新日: 2023-10-18)

主引用文献

Frick, D.N.,Virdi, R.S.,Vuksanovic, N.,Dahal, N.,Silvaggi, N.R.
Molecular Basis for ADP-Ribose Binding to the Mac1 Domain of SARS-CoV-2 nsp3.
Biochemistry, 59:2608-2615, 2020

PubMed Abstract: The virus that causes COVID-19, SARS-CoV-2, has a large RNA genome that encodes numerous proteins that might be targets for antiviral drugs. Some of these proteins, such as the RNA-dependent RNA polymerase, helicase, and main protease, are well conserved between SARS-CoV-2 and the original SARS virus, but several others are not. This study examines one of the proteins encoded by SARS-CoV-2 that is most different, a macrodomain of nonstructural protein 3 (nsp3). Although 26% of the amino acids in this SARS-CoV-2 macrodomain differ from those observed in other coronaviruses, biochemical and structural data reveal that the protein retains the ability to bind ADP-ribose, which is an important characteristic of beta coronaviruses and a potential therapeutic target.

PubMed: 32578982
DOI: 10.1021/acs.biochem.0c00309

実験手法

X-RAY DIFFRACTION (0.95 Å)