6WEX

Crystal Structure of Broadly Neutralizing Antibody 3I14-D93N Mutant Bound to the Influenza A H6 Hemagglutinin

6WEX の概要

• エントリーDOI: 10.2210/pdb6wex/pdb
• 分子名称: Hemagglutinin, Hemagglutinin HA2 chain, heavy chain, ... (5 entities in total)
• 機能のキーワード: antibody, influenza, hemagglutinin, stem epitope, viral protein, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Influenza A virus; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 109265.24

構造登録者

Harshbarger, W.D.,Lockbaum, G.J.,Deming, D.T.,Attatippaholkun, N.,Schiffer, C.A.,Marasco, W.A. (登録日: 2020-04-03, 公開日: 2020-11-25, 最終更新日: 2024-10-16)

主引用文献

Harshbarger, W.D.,Deming, D.,Lockbaum, G.J.,Attatippaholkun, N.,Kamkaew, M.,Hou, S.,Somasundaran, M.,Wang, J.P.,Finberg, R.W.,Zhu, Q.K.,Schiffer, C.A.,Marasco, W.A.
Unique structural solution from a V H 3-30 antibody targeting the hemagglutinin stem of influenza A viruses.
Nat Commun, 12:559-559, 2021

PubMed Abstract: Broadly neutralizing antibodies (bnAbs) targeting conserved influenza A virus (IAV) hemagglutinin (HA) epitopes can provide valuable information for accelerating universal vaccine designs. Here, we report structural details for heterosubtypic recognition of HA from circulating and emerging IAVs by the human antibody 3I14. Somatic hypermutations play a critical role in shaping the HCDR3, which alone and uniquely among V3-30 derived antibodies, forms contacts with five sub-pockets within the HA-stem hydrophobic groove. 3I14 light-chain interactions are also key for binding HA and contribute a large buried surface area spanning two HA protomers. Comparison of 3I14 to bnAbs from several defined classes provide insights to the bias selection of V3-30 antibodies and reveals that 3I14 represents a novel structural solution within the V3-30 repertoire. The structures reported here improve our understanding of cross-group heterosubtypic binding activity, providing the basis for advancing immunogen designs aimed at eliciting a broadly protective response to IAV.

PubMed: 33495478
DOI: 10.1038/s41467-020-20879-6

実験手法

X-RAY DIFFRACTION (3.49 Å)