6WEB

Multi-Hit SFX using MHz XFEL sources

6WEB の概要

• エントリーDOI: 10.2210/pdb6web/pdb
• 分子名称: Lysozyme, ACETATE ION, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: enzyme, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14487.72

構造登録者

Holmes, S.,Darmanin, C.,Abbey, B. (登録日: 2020-04-01, 公開日: 2021-10-13, 最終更新日: 2024-10-23)

主引用文献

Holmes, S.,Kirkwood, H.J.,Bean, R.,Giewekemeyer, K.,Martin, A.V.,Hadian-Jazi, M.,Wiedorn, M.O.,Oberthur, D.,Marman, H.,Adriano, L.,Al-Qudami, N.,Bajt, S.,Barak, I.,Bari, S.,Bielecki, J.,Brockhauser, S.,Coleman, M.A.,Cruz-Mazo, F.,Danilevski, C.,Dorner, K.,Ganan-Calvo, A.M.,Graceffa, R.,Fanghor, H.,Heymann, M.,Frank, M.,Kaukher, A.,Kim, Y.,Kobe, B.,Knoska, J.,Laurus, T.,Letrun, R.,Maia, L.,Messerschmidt, M.,Metz, M.,Michelat, T.,Mills, G.,Molodtsov, S.,Monteiro, D.C.F.,Morgan, A.J.,Munnich, A.,Pena Murillo, G.E.,Previtali, G.,Round, A.,Sato, T.,Schubert, R.,Schulz, J.,Shelby, M.,Seuring, C.,Sellberg, J.A.,Sikorski, M.,Silenzi, A.,Stern, S.,Sztuk-Dambietz, J.,Szuba, J.,Trebbin, M.,Vagovic, P.,Ve, T.,Weinhausen, B.,Wrona, K.,Xavier, P.L.,Xu, C.,Yefanov, O.,Nugent, K.A.,Chapman, H.N.,Mancuso, A.P.,Barty, A.,Abbey, B.,Darmanin, C.
Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers.
Nat Commun, 13:4708-4708, 2022

PubMed Abstract: The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by <1 µs, yield up to 2.1 Å resolution structures. Comparisons between the two structures reveal no indications of radiation damage or significant changes within the active site, consistent with the calculated dose estimates. This demonstrates MHz SFX can be used as a tool for tracking sub-microsecond structural changes in individual single crystals, a technique we refer to as multi-hit SFX.

PubMed: 35953469
DOI: 10.1038/s41467-022-32434-6

実験手法

X-RAY DIFFRACTION (2.1 Å)