6W8Y

Co-crystal structures of CHIKV nsP3 macrodomain with pyrimidone fragments

Summary for 6W8Y

• Entry DOI: 10.2210/pdb6w8y/pdb
• Descriptor: Nonstructural polyprotein, 2-oxo-2,3-dihydropyrimidine-4-carboxylic acid (3 entities in total)
• Functional Keywords: nsp3, macrodomain, pyrimidone fragments, viral protein
• Biological source: Chikungunya virus (CHIKV)
• Total number of polymer chains: 4
• Total formula weight: 74635.90

Authors

Zhang, S.,Garzan, A.,Pathak, A.K.,Augelli-Szafran, C.E.,Wu, M. (deposition date: 2020-03-21, release date: 2021-01-13, Last modification date: 2023-10-18)

Primary citation

Zhang, S.,Garzan, A.,Haese, N.,Bostwick, R.,Martinez-Gzegozewska, Y.,Rasmussen, L.,Streblow, D.N.,Haise, M.T.,Pathak, A.K.,Augelli-Szafran, C.E.,Wu, M.
Pyrimidone inhibitors targeting Chikungunya Virus nsP3 macrodomain by fragment-based drug design.
Plos One, 16:e0245013-e0245013, 2021

PubMed Abstract: The macrodomain of nsP3 (nsP3MD) is highly conserved among the alphaviruses and ADP-ribosylhydrolase activity of Chikungunya Virus (CHIKV) nsP3MD is critical for CHIKV viral replication and virulence. No small molecule drugs targeting CHIKV nsP3 have been identified to date. Here we report small fragments that bind to nsP3MD which were discovered by virtually screening a fragment library and X-ray crystallography. These identified fragments share a similar scaffold, 2-pyrimidone-4-carboxylic acid, and are specifically bound to the ADP-ribose binding site of nsP3MD. Among the fragments, 2-oxo-5,6-benzopyrimidine-4-carboxylic acid showed anti-CHIKV activity with an IC50 of 23 μM. Our fragment-based drug discovery approach provides valuable information to further develop a specific and potent nsP3 inhibitor of CHIKV viral replication based on the 2-pyrimidone-4-carboxylic acid scaffold. In silico studies suggest this pyrimidone scaffold could also bind to the macrodomains of other alphaviruses and coronaviruses and thus, have potential pan-antiviral activity.

PubMed: 33482665
DOI: 10.1371/journal.pone.0245013

Experimental method

X-RAY DIFFRACTION (2.1 Å)