6W8U

Cryo-EM of the Pyrobaculum arsenaticum pilus

6W8U の概要

• エントリーDOI: 10.2210/pdb6w8u/pdb
• EMDBエントリー: 21578
• 分子名称: pilin (1 entity in total)
• 機能のキーワード: helical symmetry, archaeal pilus, type iv pilus, protein fibril
• 由来する生物種: Pyrobaculum arsenaticum
• タンパク質・核酸の鎖数: 41
• 化学式量合計: 594116.24

構造登録者

Wang, F.,Baquero, D.P.,Su, Z.,Beltran, L.C.,Prangishvili, D.,Krupovic, M.,Egelman, E.H. (登録日: 2020-03-21, 公開日: 2020-07-08, 最終更新日: 2024-10-16)

主引用文献

Wang, F.,Baquero, D.P.,Su, Z.,Beltran, L.C.,Prangishvili, D.,Krupovic, M.,Egelman, E.H.
The structures of two archaeal type IV pili illuminate evolutionary relationships.
Nat Commun, 11:3424-3424, 2020

PubMed Abstract: We have determined the cryo-electron microscopic (cryo-EM) structures of two archaeal type IV pili (T4P), from Pyrobaculum arsenaticum and Saccharolobus solfataricus, at 3.8 Å and 3.4 Å resolution, respectively. This triples the number of high resolution archaeal T4P structures, and allows us to pinpoint the evolutionary divergence of bacterial T4P, archaeal T4P and archaeal flagellar filaments. We suggest that extensive glycosylation previously observed in T4P of Sulfolobus islandicus is a response to an acidic environment, as at even higher temperatures in a neutral environment much less glycosylation is present for Pyrobaculum than for Sulfolobus and Saccharolobus pili. Consequently, the Pyrobaculum filaments do not display the remarkable stability of the Sulfolobus filaments in vitro. We identify the Saccharolobus and Pyrobaculum T4P as host receptors recognized by rudivirus SSRV1 and tristromavirus PFV2, respectively. Our results illuminate the evolutionary relationships among bacterial and archaeal T4P filaments and provide insights into archaeal virus-host interactions.

PubMed: 32647180
DOI: 10.1038/s41467-020-17268-4

実験手法

ELECTRON MICROSCOPY (3.8 Å)