6W2V

Junction 23, DHR14-DHR18

6W2V の概要

• エントリーDOI: 10.2210/pdb6w2v/pdb
• 関連するPDBエントリー: 6w2q 6w2r
• 分子名称: Junction 23 DHR14-DHR18 (2 entities in total)
• 機能のキーワード: helical bundle, designed helical repeat, computational design, biosynthetic protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51430.11

構造登録者

Bick, M.J.,Brunette, T.J.,Baker, D. (登録日: 2020-03-08, 公開日: 2020-04-15, 最終更新日: 2024-11-06)

主引用文献

Brunette, T.J.,Bick, M.J.,Hansen, J.M.,Chow, C.M.,Kollman, J.M.,Baker, D.
Modular repeat protein sculpting using rigid helical junctions.
Proc.Natl.Acad.Sci.USA, 117:8870-8875, 2020

PubMed Abstract: The ability to precisely design large proteins with diverse shapes would enable applications ranging from the design of protein binders that wrap around their target to the positioning of multiple functional sites in specified orientations. We describe a protein backbone design method for generating a wide range of rigid fusions between helix-containing proteins and use it to design 75,000 structurally unique junctions between monomeric and homo-oligomeric de novo designed and ankyrin repeat proteins (RPs). Of the junction designs that were experimentally characterized, 82% have circular dichroism and solution small-angle X-ray scattering profiles consistent with the design models and are stable at 95 °C. Crystal structures of four designed junctions were in close agreement with the design models with rmsds ranging from 0.9 to 1.6 Å. Electron microscopic images of extended tetrameric structures and ∼10-nm-diameter "L" and "V" shapes generated using the junctions are close to the design models, demonstrating the control the rigid junctions provide for protein shape sculpting over multiple nanometer length scales.

PubMed: 32245816
DOI: 10.1073/pnas.1908768117

実験手法

X-RAY DIFFRACTION (2.399 Å)