6W1O

RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution

これはPDB形式変換不可エントリーです。

6W1O の概要

• エントリーDOI: 10.2210/pdb6w1o/pdb
• 分子名称: Photosystem II protein D1 1, Photosystem II reaction center protein K, Photosystem II reaction center protein L, ... (35 entities in total)
• 機能のキーワード: water oxidation, membrane protein, light harvesting, electron transfer, photosynthesis
• 由来する生物種: Thermosynechococcus elongatus (strain BP-1); 詳細
• タンパク質・核酸の鎖数: 40
• 化学式量合計: 754294.50

構造登録者

Ibrahim, M.,Fransson, T.,Chatterjee, R.,Cheah, M.H.,Hussein, R.,Lassalle, L.,Sutherlin, K.D.,Young, I.D.,Fuller, F.D.,Gul, S.,Kim, I.-S.,Simon, P.S.,de Lichtenberg, C.,Chernev, P.,Bogacz, I.,Pham, C.,Orville, A.M.,Saichek, N.,Northen, T.R.,Batyuk, A.,Carbajo, S.,Alonso-Mori, R.,Tono, K.,Owada, S.,Bhowmick, A.,Bolotovski, R.,Mendez, D.,Moriarty, N.W.,Holton, J.M.,Dobbek, H.,Brewster, A.S.,Adams, P.D.,Sauter, N.K.,Bergmann, U.,Zouni, A.,Messinger, J.,Kern, J.,Yachandra, V.K.,Yano, J. (登録日: 2020-03-04, 公開日: 2020-05-20, 最終更新日: 2024-10-23)

主引用文献

Ibrahim, M.,Fransson, T.,Chatterjee, R.,Cheah, M.H.,Hussein, R.,Lassalle, L.,Sutherlin, K.D.,Young, I.D.,Fuller, F.D.,Gul, S.,Kim, I.S.,Simon, P.S.,de Lichtenberg, C.,Chernev, P.,Bogacz, I.,Pham, C.C.,Orville, A.M.,Saichek, N.,Northen, T.,Batyuk, A.,Carbajo, S.,Alonso-Mori, R.,Tono, K.,Owada, S.,Bhowmick, A.,Bolotovsky, R.,Mendez, D.,Moriarty, N.W.,Holton, J.M.,Dobbek, H.,Brewster, A.S.,Adams, P.D.,Sauter, N.K.,Bergmann, U.,Zouni, A.,Messinger, J.,Kern, J.,Yachandra, V.K.,Yano, J.
Untangling the sequence of events during the S2→ S3transition in photosystem II and implications for the water oxidation mechanism.
Proc.Natl.Acad.Sci.USA, 117:12624-12635, 2020

PubMed Abstract: In oxygenic photosynthesis, light-driven oxidation of water to molecular oxygen is carried out by the oxygen-evolving complex (OEC) in photosystem II (PS II). Recently, we reported the room-temperature structures of PS II in the four (semi)stable S-states, S, S, S, and S, showing that a water molecule is inserted during the S → S transition, as a new bridging O(H)-ligand between Mn1 and Ca. To understand the sequence of events leading to the formation of this last stable intermediate state before O formation, we recorded diffraction and Mn X-ray emission spectroscopy (XES) data at several time points during the S → S transition. At the electron acceptor site, changes due to the two-electron redox chemistry at the quinones, Q and Q, are observed. At the donor site, tyrosine Y and His190 H-bonded to it move by 50 µs after the second flash, and Glu189 moves away from Ca. This is followed by Mn1 and Mn4 moving apart, and the insertion of O(H) at the open coordination site of Mn1. This water, possibly a ligand of Ca, could be supplied via a "water wheel"-like arrangement of five waters next to the OEC that is connected by a large channel to the bulk solvent. XES spectra show that Mn oxidation (τ of ∼350 µs) during the S → S transition mirrors the appearance of O electron density. This indicates that the oxidation state change and the insertion of water as a bridging atom between Mn1 and Ca are highly correlated.

PubMed: 32434915
DOI: 10.1073/pnas.2000529117

実験手法

X-RAY DIFFRACTION (2.08 Å)