6VYL

Cryo-EM structure of mechanosensitive channel MscS in PC-10 nanodiscs

6VYL の概要

• エントリーDOI: 10.2210/pdb6vyl/pdb
• EMDBエントリー: 21463
• 分子名称: Mechanosensitive channel MscS (1 entity in total)
• 機能のキーワード: mechanosensitive channel, mscs, nanodiscs, lipid, membrane protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 216460.29

構造登録者

Zhang, Y.,Daday, C.,Gu, R.,Cox, C.D.,Martinac, B.,Groot, B.,Walz, T. (登録日: 2020-02-27, 公開日: 2021-02-10, 最終更新日: 2024-03-06)

主引用文献

Zhang, Y.,Daday, C.,Gu, R.X.,Cox, C.D.,Martinac, B.,de Groot, B.L.,Walz, T.
Visualization of the mechanosensitive ion channel MscS under membrane tension.
Nature, 590:509-514, 2021

PubMed Abstract: Mechanosensitive channels sense mechanical forces in cell membranes and underlie many biological sensing processes. However, how exactly they sense mechanical force remains under investigation. The bacterial mechanosensitive channel of small conductance, MscS, is one of the most extensively studied mechanosensitive channels, but how it is regulated by membrane tension remains unclear, even though the structures are known for its open and closed states. Here we used cryo-electron microscopy to determine the structure of MscS in different membrane environments, including one that mimics a membrane under tension. We present the structures of MscS in the subconducting and desensitized states, and demonstrate that the conformation of MscS in a lipid bilayer in the open state is dynamic. Several associated lipids have distinct roles in MscS mechanosensation. Pore lipids are necessary to prevent ion conduction in the closed state. Gatekeeper lipids stabilize the closed conformation and dissociate with membrane tension, allowing the channel to open. Pocket lipids in a solvent-exposed pocket between subunits are pulled out under sustained tension, allowing the channel to transition to the subconducting state and then to the desensitized state. Our results provide a mechanistic underpinning and expand on the 'force-from-lipids' model for MscS mechanosensation.

PubMed: 33568813
DOI: 10.1038/s41586-021-03196-w

実験手法

ELECTRON MICROSCOPY (3.4 Å)