6VYD

Terpenoid Cyclase FgGS in Complex with Mg, Inorganic Pyrophosphate, and Benzyltriethylammonium cation

6VYD の概要

• エントリーDOI: 10.2210/pdb6vyd/pdb
• 分子名称: Terpenoid cyclase FgGS, MAGNESIUM ION, SULFATE ION, ... (9 entities in total)
• 機能のキーワード: terpene, terpenoid, terpenoid cyclase, lyase
• 由来する生物種: Gibberella zeae (strain PH-1 / ATCC MYA-4620 / FGSC 9075 / NRRL 31084) (Wheat head blight fungus)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 74998.16

構造登録者

Herbst-Gervasoni, C.J.,Christianson, D.W. (登録日: 2020-02-26, 公開日: 2020-07-08, 最終更新日: 2024-11-13)

主引用文献

He, H.,Bian, G.,Herbst-Gervasoni, C.J.,Mori, T.,Shinsky, S.A.,Hou, A.,Mu, X.,Huang, M.,Cheng, S.,Deng, Z.,Christianson, D.W.,Abe, I.,Liu, T.
Discovery of the cryptic function of terpene cyclases as aromatic prenyltransferases.
Nat Commun, 11:3958-3958, 2020

PubMed Abstract: Catalytic versatility is an inherent property of many enzymes. In nature, terpene cyclases comprise the foundation of molecular biodiversity as they generate diverse hydrocarbon scaffolds found in thousands of terpenoid natural products. Here, we report that the catalytic activity of the terpene cyclases AaTPS and FgGS can be switched from cyclase to aromatic prenyltransferase at basic pH to generate prenylindoles. The crystal structures of AaTPS and FgGS provide insights into the catalytic mechanism of this cryptic function. Moreover, aromatic prenyltransferase activity discovered in other terpene cyclases indicates that this cryptic function is broadly conserved among the greater family of terpene cyclases. We suggest that this cryptic function is chemoprotective for the cell by regulating isoprenoid diphosphate concentrations so that they are maintained below toxic thresholds.

PubMed: 32769971
DOI: 10.1038/s41467-020-17642-2

実験手法

X-RAY DIFFRACTION (1.46 Å)