6VSB

Prefusion 2019-nCoV spike glycoprotein with a single receptor-binding domain up

6VSB の概要

• エントリーDOI: 10.2210/pdb6vsb/pdb
• EMDBエントリー: 21374 21375
• 分子名称: Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: spike, fusion glycoprotein, coronavirus, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 440385.56

構造登録者

Wrapp, D.,Wang, N.,Corbett, K.S.,Goldsmith, J.A.,Hsieh, C.,Abiona, O.,Graham, B.S.,McLellan, J.S. (登録日: 2020-02-10, 公開日: 2020-02-26, 最終更新日: 2024-10-23)

主引用文献

Wrapp, D.,Wang, N.,Corbett, K.S.,Goldsmith, J.A.,Hsieh, C.L.,Abiona, O.,Graham, B.S.,McLellan, J.S.
Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation.
Science, 367:1260-1263, 2020

PubMed Abstract: The outbreak of a novel coronavirus (2019-nCoV) represents a pandemic threat that has been declared a public health emergency of international concern. The CoV spike (S) glycoprotein is a key target for vaccines, therapeutic antibodies, and diagnostics. To facilitate medical countermeasure development, we determined a 3.5-angstrom-resolution cryo-electron microscopy structure of the 2019-nCoV S trimer in the prefusion conformation. The predominant state of the trimer has one of the three receptor-binding domains (RBDs) rotated up in a receptor-accessible conformation. We also provide biophysical and structural evidence that the 2019-nCoV S protein binds angiotensin-converting enzyme 2 (ACE2) with higher affinity than does severe acute respiratory syndrome (SARS)-CoV S. Additionally, we tested several published SARS-CoV RBD-specific monoclonal antibodies and found that they do not have appreciable binding to 2019-nCoV S, suggesting that antibody cross-reactivity may be limited between the two RBDs. The structure of 2019-nCoV S should enable the rapid development and evaluation of medical countermeasures to address the ongoing public health crisis.

PubMed: 32075877
DOI: 10.1126/science.abb2507

実験手法

ELECTRON MICROSCOPY (3.46 Å)