6VRC

Cryo-EM structure of Cas13(crRNA)

6VRC の概要

• エントリーDOI: 10.2210/pdb6vrc/pdb
• EMDBエントリー: 21366 21367
• 分子名称: CRISPR-associated endoribonuclease Cas13a, RNA (51-MER) (2 entities in total)
• 機能のキーワード: crispr-cas system, cas13, immune system, typevi
• 由来する生物種: Listeria seeligeri serovar 1/2b (strain ATCC 35967 / DSM 20751 / CIP 100100 / SLCC 3954); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 152344.68

構造登録者

Jia, N.,Meeske, A.J.,Marraffini, L.A.,Patel, D.J. (登録日: 2020-02-07, 公開日: 2020-06-10, 最終更新日: 2024-03-06)

主引用文献

Meeske, A.J.,Jia, N.,Cassel, A.K.,Kozlova, A.,Liao, J.,Wiedmann, M.,Patel, D.J.,Marraffini, L.A.
A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity.
Science, 369:54-59, 2020

PubMed Abstract: The CRISPR RNA (crRNA)-guided nuclease Cas13 recognizes complementary viral transcripts to trigger the degradation of both host and viral RNA during the type VI CRISPR-Cas antiviral response. However, how viruses can counteract this immunity is not known. We describe a listeriaphage (ϕLS46) encoding an anti-CRISPR protein (AcrVIA1) that inactivates the type VI-A CRISPR system of Using genetics, biochemistry, and structural biology, we found that AcrVIA1 interacts with the guide-exposed face of Cas13a, preventing access to the target RNA and the conformational changes required for nuclease activation. Unlike inhibitors of DNA-cleaving Cas nucleases, which cause limited immunosuppression and require multiple infections to bypass CRISPR defenses, a single dose of AcrVIA1 delivered by an individual virion completely dismantles type VI-A CRISPR-mediated immunity.

PubMed: 32467331
DOI: 10.1126/science.abb6151

実験手法

ELECTRON MICROSCOPY (3.2 Å)