6VOZ

Artificial Metalloproteins with Dinuclear Iron Centers

6VOZ の概要

• エントリーDOI: 10.2210/pdb6voz/pdb
• 分子名称: Streptavidin, {N-(4-{bis[(pyridin-2-yl-kappaN)methyl]amino-kappaN}butyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}iron(3+), ACETATE ION, ... (4 entities in total)
• 機能のキーワード: biotin binding artificial metalloprotein, metal binding protein
• 由来する生物種: Streptomyces avidinii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17173.49

構造登録者

Miller, K.R.,Follmer, A.H.,Jasniewski, A.J.,Sabuncu, S.,Biswas, S.,Albert, T.,Hendrich, M.P.,Moenne-Loccoz, P.,Borovik, A.S. (登録日: 2020-02-01, 公開日: 2021-02-03, 最終更新日: 2023-10-11)

主引用文献

Miller, K.R.,Biswas, S.,Jasniewski, A.,Follmer, A.H.,Biswas, A.,Albert, T.,Sabuncu, S.,Bominaar, E.L.,Hendrich, M.P.,Moenne-Loccoz, P.,Borovik, A.S.
Artificial Metalloproteins with Dinuclear Iron-Hydroxido Centers.
J.Am.Chem.Soc., 143:2384-2393, 2021

PubMed Abstract: Dinuclear iron centers with a bridging hydroxido or oxido ligand form active sites within a variety of metalloproteins. A key feature of these sites is the ability of the protein to control the structures around the Fe centers, which leads to entatic states that are essential for function. To simulate this controlled environment, artificial proteins have been engineered using biotin-streptavidin (Sav) technology in which Fe complexes from adjacent subunits can assemble to form [Fe-(μ-OH)-Fe] cores. The assembly process is promoted by the site-specific localization of the Fe complexes within a subunit through the designed mutation of a tyrosinate side chain to coordinate the Fe centers. An important outcome is that the Sav host can regulate the Fe···Fe separation, which is known to be important for function in natural metalloproteins. Spectroscopic and structural studies from X-ray diffraction methods revealed uncommonly long Fe···Fe separations that change by less than 0.3 Å upon the binding of additional bridging ligands. The structural constraints imposed by the protein host on the di-Fe cores are unique and create examples of active sites having entatic states within engineered artificial metalloproteins.

PubMed: 33528256
DOI: 10.1021/jacs.0c12564

実験手法

X-RAY DIFFRACTION (1.3 Å)