6VOA

Cryo-EM structure of the BBSome-ARL6 complex

6VOA の概要

• エントリーDOI: 10.2210/pdb6voa/pdb
• EMDBエントリー: 21259
• 分子名称: Bardet-Biedl syndrome 18 protein, Bardet-Biedl syndrome 2 protein homolog, Bardet-Biedl syndrome 4 protein homolog, ... (9 entities in total)
• 機能のキーワード: cilia, bardet-biedl syndrome, protein transport
• 由来する生物種: Bos taurus (Bovine); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 507566.42

構造登録者

Yang, S.,Walz, T.,Nachury, M.V. (登録日: 2020-01-30, 公開日: 2020-06-24, 最終更新日: 2024-03-06)

主引用文献

Yang, S.,Bahl, K.,Chou, H.T.,Woodsmith, J.,Stelzl, U.,Walz, T.,Nachury, M.V.
Near-atomic structures of the BBSome reveal the basis for BBSome activation and binding to GPCR cargoes.
Elife, 9:-, 2020

PubMed Abstract: Dynamic trafficking of G protein-coupled receptors (GPCRs) out of cilia is mediated by the BBSome. In concert with its membrane recruitment factor, the small GTPase ARL6/BBS3, the BBSome ferries GPCRs across the transition zone, a diffusion barrier at the base of cilia. Here, we present the near-atomic structures of the BBSome by itself and in complex with ARL6, and we describe the changes in BBSome conformation induced by ARL6 binding. Modeling the interactions of the BBSome with membranes and the GPCR Smoothened (SMO) reveals that SMO, and likely also other GPCR cargoes, must release their amphipathic helix 8 from the membrane to be recognized by the BBSome.

PubMed: 32510327
DOI: 10.7554/eLife.55954

実験手法

ELECTRON MICROSCOPY (4 Å)