6VMI の概要
| エントリーDOI | 10.2210/pdb6vmi/pdb |
| EMDBエントリー | 21233 21242 |
| 分子名称 | 12s rRNA, 28S ribosomal protein S18b, mitochondrial, 28S ribosomal protein S18c, mitochondrial, ... (92 entities in total) |
| 機能のキーワード | mitochondrial elongation factor-g1, 55s ribosome, ribosome |
| 由来する生物種 | Homo sapiens (Human) 詳細 |
| タンパク質・核酸の鎖数 | 91 |
| 化学式量合計 | 3077924.61 |
| 構造登録者 | |
| 主引用文献 | Koripella, R.K.,Sharma, M.R.,Bhargava, K.,Datta, P.P.,Kaushal, P.S.,Keshavan, P.,Spremulli, L.L.,Banavali, N.K.,Agrawal, R.K. Structures of the human mitochondrial ribosome bound to EF-G1 reveal distinct features of mitochondrial translation elongation. Nat Commun, 11:3830-3830, 2020 Cited by PubMed Abstract: The mammalian mitochondrial ribosome (mitoribosome) and its associated translational factors have evolved to accommodate greater participation of proteins in mitochondrial translation. Here we present the 2.68-3.96 Å cryo-EM structures of the human 55S mitoribosome in complex with the human mitochondrial elongation factor G1 (EF-G1) in three distinct conformational states, including an intermediate state and a post-translocational state. These structures reveal the role of several mitochondria-specific (mito-specific) mitoribosomal proteins (MRPs) and a mito-specific segment of EF-G1 in mitochondrial tRNA (tRNA) translocation. In particular, the mito-specific C-terminal extension in EF-G1 is directly involved in translocation of the acceptor arm of the A-site tRNA. In addition to the ratchet-like and independent head-swiveling motions exhibited by the small mitoribosomal subunit, we discover significant conformational changes in MRP mL45 at the nascent polypeptide-exit site within the large mitoribosomal subunit that could be critical for tethering of the elongating mitoribosome onto the inner-mitochondrial membrane. PubMed: 32737313DOI: 10.1038/s41467-020-17715-2 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (2.96 Å) |
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