6VKX

Crystal structure of the carbohydrate-binding domain VP8* of human P[8] rotavirus strain BM13851

6VKX の概要

• エントリーDOI: 10.2210/pdb6vkx/pdb
• 分子名称: Outer capsid protein VP4, TETRAETHYLENE GLYCOL, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: rotavirus, host receptor interaction, viral protein
• 由来する生物種: Human rotavirus A
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 55613.04

構造登録者

Xu, S.,McGinnis, K.R.,Jiang, X.,Kennedy, M.A. (登録日: 2020-01-22, 公開日: 2021-01-27, 最終更新日: 2023-10-11)

主引用文献

Xu, S.,McGinnis, K.R.,Liu, Y.,Huang, P.,Tan, M.,Stuckert, M.R.,Burnside, R.E.,Jacob, E.G.,Ni, S.,Jiang, X.,Kennedy, M.A.
Structural basis of P[II] rotavirus evolution and host ranges under selection of histo-blood group antigens.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: Group A rotaviruses cause severe gastroenteritis in infants and young children worldwide, with P[II] genogroup rotaviruses (RVs) responsible for >90% of global cases. RVs have diverse host ranges in different human and animal populations determined by host histo-blood group antigen (HBGA) receptor polymorphism, but details governing diversity, host ranges, and species barriers remain elusive. In this study, crystal structures of complexes of the major P[II] genogroup P[4] and P[8] genotype RV VP8* receptor-binding domains together with Lewis epitope-containing LNDFH I glycans in combination with VP8* receptor-glycan ligand affinity measurements based on NMR titration experiments revealed the structural basis for RV genotype-specific switching between ββ and βα HBGA receptor-binding sites that determine RV host ranges. The data support the hypothesis that P[II] RV evolution progressed from animals to humans under the selection of type 1 HBGAs guided by stepwise host synthesis of type 1 ABH and Lewis HBGAs. The results help explain disease burden, species barriers, epidemiology, and limited efficacy of current RV vaccines in developing countries. The structural data has the potential to impact the design of future vaccine strategies against RV gastroenteritis.

PubMed: 34475219
DOI: 10.1073/pnas.2107963118

実験手法

X-RAY DIFFRACTION (1.71 Å)