6VHC
1.4A damaged structure of GSNQNNF used to determine initial phases from radiation damage
Summary for 6VHC
| Entry DOI | 10.2210/pdb6vhc/pdb |
| Related | 6clj |
| EMDB information | 21203 |
| Descriptor | GSNQNNF, ZINC ION, ACETATE ION, ... (4 entities in total) |
| Functional Keywords | microed, damage, phasing, rip, protein fibril |
| Biological source | synthetic construct |
| Total number of polymer chains | 1 |
| Total formula weight | 904.21 |
| Authors | Martynowycz, M.W.,Hattne, J.,Gonen, T. (deposition date: 2020-01-09, release date: 2020-02-19, Last modification date: 2024-03-06) |
| Primary citation | Martynowycz, M.W.,Hattne, J.,Gonen, T. Experimental Phasing of MicroED Data Using Radiation Damage. Structure, 28:458-, 2020 Cited by PubMed Abstract: We previously demonstrated that microcrystal electron diffraction (MicroED) can be used to determine atomic-resolution structures from vanishingly small three-dimensional crystals. Here, we present an example of an experimentally phased structure using only MicroED data. The structure of a seven-residue peptide is solved starting from differences to the diffraction intensities induced by structural changes due to radiation damage. The same wedge of reciprocal space was recorded twice by continuous-rotation MicroED from a set of 11 individual crystals. The data from the first pass were merged to make a "low-dose dataset." The data from the second pass were similarly merged to form a "damaged dataset." Differences between these two datasets were used to identify a single heavy-atom site from a Patterson difference map, and initial phases were generated. Finally, the structure was completed by iterative cycles of modeling and refinement. PubMed: 32023481DOI: 10.1016/j.str.2020.01.008 PDB entries with the same primary citation |
| Experimental method | ELECTRON CRYSTALLOGRAPHY (1.4 Å) |
Structure validation
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