6VHC

1.4A damaged structure of GSNQNNF used to determine initial phases from radiation damage

6VHC の概要

• エントリーDOI: 10.2210/pdb6vhc/pdb
• 関連するPDBエントリー: 6clj
• EMDBエントリー: 21203
• 分子名称: GSNQNNF, ZINC ION, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: microed, damage, phasing, rip, protein fibril
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 904.21

構造登録者

Martynowycz, M.W.,Hattne, J.,Gonen, T. (登録日: 2020-01-09, 公開日: 2020-02-19, 最終更新日: 2024-03-06)

主引用文献

Martynowycz, M.W.,Hattne, J.,Gonen, T.
Experimental Phasing of MicroED Data Using Radiation Damage.
Structure, 28:458-, 2020

PubMed Abstract: We previously demonstrated that microcrystal electron diffraction (MicroED) can be used to determine atomic-resolution structures from vanishingly small three-dimensional crystals. Here, we present an example of an experimentally phased structure using only MicroED data. The structure of a seven-residue peptide is solved starting from differences to the diffraction intensities induced by structural changes due to radiation damage. The same wedge of reciprocal space was recorded twice by continuous-rotation MicroED from a set of 11 individual crystals. The data from the first pass were merged to make a "low-dose dataset." The data from the second pass were similarly merged to form a "damaged dataset." Differences between these two datasets were used to identify a single heavy-atom site from a Patterson difference map, and initial phases were generated. Finally, the structure was completed by iterative cycles of modeling and refinement.

PubMed: 32023481
DOI: 10.1016/j.str.2020.01.008

実験手法

ELECTRON CRYSTALLOGRAPHY (1.4 Å)