6VD9

Metal-bound C-terminal domain of the CzcD transporter from Cuprividus metallidurans

6VD9 の概要

• エントリーDOI: 10.2210/pdb6vd9/pdb
• 分子名称: Metal cation efflux system protein CzcD, NICKEL (II) ION (3 entities in total)
• 機能のキーワード: cation diffusion facilitator protein (cdf), czcd, transport protein
• 由来する生物種: Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 33684.82

構造登録者

Maher, M.J. (登録日: 2019-12-23, 公開日: 2020-06-24, 最終更新日: 2023-10-11)

主引用文献

Udagedara, S.R.,La Porta, D.M.,Spehar, C.,Purohit, G.,Hein, M.J.A.,Fatmous, M.E.,Casas Garcia, G.P.,Ganio, K.,McDevitt, C.A.,Maher, M.J.
Structural and functional characterizations of the C-terminal domains of CzcD proteins.
J.Inorg.Biochem., 208:111087-111087, 2020

PubMed Abstract: Zinc is a potent antimicrobial component of the innate immune response at the host-pathogen interface. Bacteria subvert or resist host zinc insults by metal efflux pathways that include cation diffusion facilitator (CDF) proteins. The structural and functional examination of this protein class has been limited, with only the structures of the zinc transporter YiiP proteins from E. coli and Shewanella oneidensis described to date. Here, we determine the metal binding properties, solution quaternary structures and three dimensional architectures of the C-terminal domains of the metal transporter CzcD proteins from Cupriavidus metallidurans, Pseudomonas aeruginosa and Thermotoga maritima. We reveal significant diversity in the metal-binding properties and structures of these proteins and discover a potential novel mechanism for metal-promoted dimerization for the Cupriavidus metallidurans and Pseudomonas aeruginosa proteins.

PubMed: 32505855
DOI: 10.1016/j.jinorgbio.2020.111087

実験手法

X-RAY DIFFRACTION (1.75 Å)