6V93

Structure of DNA Polymerase Zeta/DNA/dNTP Ternary Complex

6V93 の概要

• エントリーDOI: 10.2210/pdb6v93/pdb
• EMDBエントリー: 21115
• 分子名称: DNA polymerase zeta catalytic subunit, DNA polymerase zeta processivity subunit, DNA polymerase delta small subunit, ... (9 entities in total)
• 機能のキーワード: dna replication, dna repair, translesion dna synthesis, dna polymerase, dna binding protein
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 350413.75

構造登録者

Malik, R.,Kopylov, M.,Jain, R.,Ubarrextena-Belandia, I.,Aggarwal, A.K. (登録日: 2019-12-13, 公開日: 2020-08-19, 最終更新日: 2025-05-14)

主引用文献

Malik, R.,Kopylov, M.,Gomez-Llorente, Y.,Jain, R.,Johnson, R.E.,Prakash, L.,Prakash, S.,Ubarretxena-Belandia, I.,Aggarwal, A.K.
Structure and mechanism of B-family DNA polymerase zeta specialized for translesion DNA synthesis.
Nat.Struct.Mol.Biol., 27:913-924, 2020

PubMed Abstract: DNA polymerase ζ (Polζ) belongs to the same B-family as high-fidelity replicative polymerases, yet is specialized for the extension reaction in translesion DNA synthesis (TLS). Despite its importance in TLS, the structure of Polζ is unknown. We present cryo-EM structures of the Saccharomyces cerevisiae Polζ holoenzyme in the act of DNA synthesis (3.1 Å) and without DNA (4.1 Å). Polζ displays a pentameric ring-like architecture, with catalytic Rev3, accessory Pol31' Pol32 and two Rev7 subunits forming an uninterrupted daisy chain of protein-protein interactions. We also uncover the features that impose high fidelity during the nucleotide-incorporation step and those that accommodate mismatches and lesions during the extension reaction. Collectively, we decrypt the molecular underpinnings of Polζ's role in TLS and provide a framework for new cancer therapeutics.

PubMed: 32807989
DOI: 10.1038/s41594-020-0476-7

実験手法

ELECTRON MICROSCOPY (3.1 Å)