6UZJ

NMR structure of the HACS1 SH3 domain

「2KEA」から置き換えられました

6UZJ の概要

• エントリーDOI: 10.2210/pdb6uzj/pdb
• 関連するPDBエントリー: 2EBP
• NMR情報: BMRB: 16141,30684
• 分子名称: SAM domain-containing protein SAMSN-1 (1 entity in total)
• 機能のキーワード: adaptor protein sh3 domain protein-protein interaction, signaling protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9656.99

構造登録者

Donaldson, L.W. (登録日: 2019-11-15, 公開日: 2019-11-27, 最終更新日: 2024-05-15)

主引用文献

Kwan, J.J.,Slavkovic, S.,Piazza, M.,Wang, D.,Dieckmann, T.,Johnson, P.E.,Wen, X.Y.,Donaldson, L.W.
HACS1 signaling adaptor protein recognizes a motif in the paired immunoglobulin receptor B cytoplasmic domain.
Commun Biol, 3:672-672, 2020

PubMed Abstract: Hematopoietic adaptor containing SH3 and SAM domains-1 (HACS1) is a signaling protein with two juxtaposed protein-protein interaction domains and an intrinsically unstructured region that spans half the sequence. Here, we describe the interaction between the HACS1 SH3 domain and a sequence near the third immunoreceptor tyrosine-based inhibition motif (ITIM3) of the paired immunoglobulin receptor B (PIRB). From surface plasmon resonance binding assays using a mouse and human PIRB ITIM3 phosphopeptides as ligands, the HACS1 SH3 domain and SHP2 N-terminal SH2 domain demonstrated comparable affinities in the micromolar range. Since the PIRB ITIM3 sequence represents an atypical ligand for an SH3 domain, we determined the NMR structure of the HACS1 SH3 domain and performed a chemical shift mapping study. This study showed that the binding site on the HACS1 SH3 domain for PIRB shares many of the same amino acids found in a canonical binding cleft normally associated with polyproline ligands. Molecular modeling suggests that the respective binding sites in PIRB ITIM3 for the HACS1 SH3 domain and the SHP2 SH2 domain are too close to permit simultaneous binding. As a result, the HACS1-PIRB partnership has the potential to amalgamate signaling pathways that influence both immune and neuronal cell fate.

PubMed: 33188360
DOI: 10.1038/s42003-020-01397-z

実験手法

SOLUTION NMR