6UVU

Crystal structure of the AntR antimony-specific transcriptional repressor

6UVU の概要

• エントリーDOI: 10.2210/pdb6uvu/pdb
• 分子名称: ArsR family transcriptional regulator (2 entities in total)
• 機能のキーワード: arsr, arsenic/antimony binding, transcriptional repressor, transcription
• 由来する生物種: Comamonas testosteroni
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27069.64

構造登録者

Thiruselvam, V.,Banumathi, S.,Palani, K.,Manohar, R.,Rosen, B.P. (登録日: 2019-11-04, 公開日: 2020-11-04, 最終更新日: 2023-10-11)

主引用文献

Viswanathan, T.,Chen, J.,Wu, M.,An, L.,Kandavelu, P.,Sankaran, B.,Radhakrishnan, M.,Li, M.,Rosen, B.P.
Functional and structural characterization of AntR, an Sb(III) responsive transcriptional repressor.
Mol.Microbiol., 116:427-437, 2021

PubMed Abstract: The ant operon of the antimony-mining bacterium Comamonas testosterone JL40 confers resistance to Sb(III). The operon is transcriptionally regulated by the product of the first gene in the operon, antR. AntR is a member of ArsR/SmtB family of metal/metalloid-responsive repressors resistance. We purified and characterized C. testosterone AntR and demonstrated that it responds to metalloids in the order Sb(III) = methylarsenite (MAs(III) >> As(III)). The protein was crystallized, and the structure was solved at 2.1 Å resolution. The homodimeric structure of AntR adopts a classical ArsR/SmtB topology architecture. The protein has five cysteine residues, of which Cys103 from one monomer and Cys113 from the other monomer, are proposed to form one Sb(III) binding site, and Cys113 and Cys103 forming a second binding site. This is the first report of the structure and binding properties of a transcriptional repressor with high selectivity for environmental antimony.

PubMed: 33786926
DOI: 10.1111/mmi.14721

実験手法

X-RAY DIFFRACTION (2.1 Å)